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Cytotoxin-Associated Gene A-Positive Helicobacter pylori Promotes Autophagy in Colon Cancer Cells by Inhibiting miR-125b-5p
Canadian Journal of Infectious Diseases and Medical Microbiology ( IF 2.8 ) Pub Date : 2021-03-02 , DOI: 10.1155/2021/6622092
Xiaolin Zhong 1, 2 , Ou Chen 3 , TieJun Zhou 4 , Muhan Lü 1, 2 , Juyi Wan 5, 6, 7
Affiliation  

Objectives. To investigate the effects of cytotoxin-associated gene A- (CagA-) positive Helicobacter pylori on proliferation, invasion, autophagy, and expression of miR-125b-5p in colon cancer cells. Methods. Colon cancer cells were cocultured with H. pylori (CagA+) to analyze the effects of H. pylori on miR-125b-5p and autophagy. Colon cancer cells infected with H. pylori (CagA+) were mimicked by transfection of CagA plasmid. The effects of CagA on the proliferation, invasion, and autophagy of colon cancer cells were analyzed. Cell counting kit-8 (CCK-8), clone formation, and Transwell assays were used to detect cell viability, proliferation, and invasion ability, respectively. Proteins and miRNAs were detected by western blotting and qPCR, respectively. Results. H. pylori (CagA+) inhibited expression of miR-125b-5p and promoted autophagy in colon cancer cells. MiR-125 b-5p was underexpressed in colon cancer cells after CagA overexpression. CagA promoted colon cancer cell proliferation, invasion, and autophagy. Overexpression of miR-125b-5p inhibited the proliferation, invasion, and autophagy of colon cancer cells and reversed the effects of CagA. Conclusion. H. pylori (CagA+) infection may promote the development and invasion of colon cancer by inhibiting miR-125b-5p.

中文翻译:

细胞毒素相关基因 A 阳性幽门螺杆菌通过抑制 miR-125b-5p 促进结肠癌细胞自噬

目标。探讨细胞毒素相关基因A-(CagA-)阳性幽门螺杆菌对结肠癌细胞增殖、侵袭、自噬及miR-125b-5p表达的影响。方法。结肠癌细胞与H. pylori (CagA+) 共培养以分析H. pylori对 miR-125b-5p 和自噬的影响。感染幽门螺杆菌的结肠癌细胞(CagA+) 通过转染 CagA 质粒进行模拟。分析了CagA对结肠癌细胞增殖、侵袭和自噬的影响。细胞计数试剂盒 8 (CCK-8)、克隆形成和 Transwell 检测分别用于检测细胞活力、增殖和侵袭能力。分别通过蛋白质印迹和 qPCR 检测蛋白质和 miRNA。结果H. pylori (CagA+) 抑制 miR-125b-5p 的表达并促进结肠癌细胞的自噬。CagA 过表达后,MiR-125 b-5p 在结肠癌细胞中表达不足。CagA 促进结肠癌细胞增殖、侵袭和自噬。miR-125b-5p 的过表达抑制了结肠癌细胞的增殖、侵袭和自噬,并逆转了 CagA 的作用。结论H. pylori (CagA+) 感染可能通过抑制 miR-125b-5p 促进结肠癌的发展和侵袭。
更新日期:2021-03-02
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