ABSTRACT

Subcutaneous adipose tissue (SAT) is recognized as a highly active metabolic and inflammatory tissue. Interestingly, adipose tissue transplantation is widely performed in plastic surgery via lipofilling, yet little is known about the gene alteration of adipocytes after transplantation. We performed an RNA-expression analysis of fat transplants before and after fat transplantation.

In C57BL/6 N mice SAT was autologously transplanted. Samples of SAT were analysed before transplantation, 7, and 15 days after transplantation and gene expression profiles were measured.

Analysis revealed that lipid metabolism-related genes were downregulated while inflammatory and extracellular matrix related genes were up-regulated 7 and 15 days after transplantation. When comparing gene expression profile 7 days after transplantation to 15 days after transplantation developmental pathways showed most changes.

摘要

皮下脂肪组织 (SAT) 被认为是一种高度活跃的代谢和炎症组织。有趣的是，脂肪组织移植通过脂肪填充在整形手术中广泛进行，但对移植后脂肪细胞的基因改变知之甚少。我们对脂肪移植前后的脂肪移植进行了 RNA 表达分析。

在 C57BL/6 N 小鼠中自体移植 SAT。在移植前、移植后 7 天和 15 天对 SAT 样本进行分析，并测量基因表达谱。

分析表明，在移植后 7 天和 15 天，脂质代谢相关基因下调，而炎症和细胞外基质相关基因上调。当比较移植后 7 天和移植后 15 天的基因表达谱时，发育途径显示出最大的变化。