In this study, five adsorption isotherm models, that is, the Langmuir, Freundlich, Langmuir–Freundlich, Temkin and Brunauer–Emmett–Teller isotherms, were utilized for the analysis of the experimental adsorption data for six classes of poly(N‐isopropylacrylamide)‐based thermo‐responsive copolymer‐grafted Sepharose Fast Flow sorbents of different copolymer compositions with two structurally related proteins, namely bovine holo‐lactoferrin and bovine holo‐transferrin at 20 and 50°C. The experimental data for bovine holo‐lactoferrin could be mathematically fitted to the Freundlich and Temkin isotherms when the protein feed concentrations were in the range of 1–40 mg/mL at both 20 and 50°C. Similar analysis of the binding of the homologous protein, bovine holo‐transferrin, to the same thermo‐responsive copolymer‐grafted sorbents revealed that the experimental data could be fitted to the Langmuir, Freundlich and Temkin isotherms with coefficients of determination value over 0.90.

中文翻译：

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蛋白质吸附到热响应聚合物接枝 Sepharose Fast Flow 吸附剂的等温模型

在本研究中，利用 Langmuir、Freundlich、Langmuir-Freundlich、Temkin 和 Brunauer-Emmett-Teller 等温线等五种吸附等温线模型对六类聚（N-异丙基丙烯酰胺）基热响应共聚物接枝琼脂糖凝胶快速流动吸附剂，在 20 和 50°C 下具有两种结构相关的蛋白质，即牛全乳铁蛋白和牛全转铁蛋白。当蛋白质饲料浓度在 20 和 50°C 下都在 1–40 mg/mL 范围内时，牛全乳铁蛋白的实验数据可以在数学上拟合 Freundlich 和 Temkin 等温线。对同源蛋白牛全转铁蛋白与相同的热响应共聚物接枝吸附剂的结合进行类似分析表明，实验数据可以拟合朗缪尔、弗罗因德里希和 Temkin 等温线，其测定系数值超过 0.90。