Appropriate soil conditions as a cultural practice are the basis of medicinal plant production. The experiment was carried out to investigate the effect of NPK, vermicompost (VC), Pseudomonas fluorescens (PF), Azotobacter chroococcum (AC), Azospirillum brasilense (AB), Glomus mosseae (AMF) on plant weight, antioxidant capacity and fatty acid (FA) profile of seed oil, and leaf nutrients of black cumin (Nigella sativa Linn) in 2018 and 2019. The results showed that dry weight in plants treated with VC (24.9 g and 28.8 g) or in AMF-inoculated plants (23.4 g and 27.8 g) was significantly higher compared with other treatments in 2018 and 2019, respectively. The highest (1.34 mg gallic acid (GA) g⁻¹ oil) total phenolic content (TPC) and DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activity (86.8 %) was observed in plants treated with VC, and followed by AMF. The main fatty acid component was linoleic acid (51.92–55.46 %), followed by oleic acid (22.6–24.5 %) and palmitic acid (13.24–16.27 %). The saturated fatty acid (SFA) under NPK application (23.39 % and 24.21 % in 2018 and 2019) was higher than other experimental treatments. Monounsaturated fatty acid (MUFA) concentration in plants inoculated with AMF (25.15 % and 25.41 % in 2018 and 2019, respectively) or supplied with AB and PF was greater. The seed oil of black cumin contained the significant level of polyunsaturated fatty acid (PUFA), which its highest content was obtained at in plants treated with VC as 55.74 % and 55.79 % in 2018 and 2019, respectively. Sustainable agricultural practices are required to increase ecofriendly performance, by using soil micro-organisms, conserving the natural resource base for improved medicinal plant cultivation.

适当的土壤条件作为一种文化习俗是药用植物生产的基础。进行了该实验以研究NPK，com粉（VC），荧光假单胞菌（PF），绿脓杆菌（AC），巴西固氮螺菌（AB），mosmose（AMF）对植物体重，抗氧化能力和脂肪酸（ FA）种子油和黑色小茴香（Nigella sativa Linn）在2018年和2019年的营养成分。结果表明，用VC（24.9 g和28.8 g）处理的植物或接种AMF的植物（23.4 g和27.8 g）分别比2018年和2019年的其他治疗方法高得多。最高（1.34 mg没食子酸（GA）g ^-1在用VC处理的植物中观察到总酚含量（TPC）和DPPH（1,1-二苯基-2-吡啶并肼基）的清除活性（86.8％），然后是AMF。脂肪酸的主要成分是亚油酸（51.92–55.46％），其次是油酸（22.6–24.5％）和棕榈酸（13.24–16.27％）。NPK施用下的饱和脂肪酸（SFA）分别高于其他实验处理（2018年和2019年分别为23.39％和24.21％）。接种AMF（分别在2018年和2019年分别为25.15％和25.41％）或由AB和PF提供的植物中的单不饱和脂肪酸（MUFA）浓度更高。黑孜然的种子油中含有大量的多不饱和脂肪酸（PUFA），在VC处理的植物中，其最高含量分别在2018年和2019年分别为55.74％和55.79％。