The increased availability and production of lignocellulosic agroindustrial wastes has originated proposals for their use as raw material to obtain biofuels (ethanol and biodiesel) or derived products. However, for biomass generated from lignocellulosic residues to be successfully degraded, in most cases it requires a physical (thermal), chemical, or enzymatic pretreatment before the application of microbial or enzymatic fermentation technologies (biocatalysis). In the context of enzymatic technologies, fungi have demonstrated to produce enzymes capable of degrading polysaccharides like cellulose, hemicelluloses and pectin. Because of this ability for degrading lignocellulosic material, researchers are making efforts to isolate and identify fungal enzymes that could have a better activity for the degradation of plant cell walls and agroindustrial biomass. We performed an in silico analysis of alpha-glucoronidase in 82 accessions of the genus Aspergillus. The constructed dendrograms of amino acid sequences defined the formation of 6 groups (I, II, III, IV, V, and VI), which demonstrates the high diversity of the enzyme. Despite this ample divergence between enzyme groups, our 3D structure modeling showed both conservation and differences in amino acid residues participating in enzyme–substrate binding, which indicates the possibility that some enzymes are functionally specialized for the specific degradation of a substrate depending on the genetics of each species in the genus and the condition of the habitat where they evolved. The identification of alpha-glucuronidase isoenzymes would allow future use of genetic engineering and biocatalysis technologies aimed at specific production of the enzyme for its use in biotransformation.

木质纤维素农业工业废物的可用性和产量的增加引发了将其用作获得生物燃料（乙醇和生物柴油）或衍生产品的原材料的提议。然而，要成功降解由木质纤维素残留物产生的生物质，在大多数情况下，它需要在应用微生物或酶促发酵技术（生物催化）之前进行物理（热）、化学或酶促预处理。在酶促技术的背景下，真菌已证明产生能够降解多糖（如纤维素、半纤维素和果胶）的酶。由于这种降解木质纤维素材料的能力，研究人员正在努力分离和鉴定真菌酶，这些酶对植物细胞壁和农业工业生物量的降解具有更好的活性。我们执行了一个82份曲霉属种质中α-葡萄糖醛酸酶的计算机模拟分析. 构建的氨基酸序列树状图定义了 6 个组（I、II、III、IV、V 和 VI）的形成，这证明了酶的高度多样性。尽管酶组之间存在很大差异，但我们的 3D 结构模型显示参与酶-底物结合的氨基酸残基的保守性和差异性，这表明某些酶可能在功能上专门用于底物的特定降解，具体取决于该属中的每个物种及其进化的栖息地条件。α-葡萄糖醛酸酶同工酶的鉴定将允许未来使用基因工程和生物催化技术，旨在特定生产用于生物转化的酶。