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A Methodology to Identify the Releasing of the Amide-Containing β-Glucan from the Usnea Lichen: A Spectroscopic Study
Journal of Polymers and the Environment ( IF 5.3 ) Pub Date : 2021-03-02 , DOI: 10.1007/s10924-021-02104-7
Rafaella F. Fernandes , Gustavo A. S. Alves , Renato V. Gonçalves , Marcia L. A. Temperini

Polymer fractions from a biological matrix have been well-characterized by vibrational spectroscopy, XPS, CP/MAS 13C NMR, MALDI-TOF mass spectrometry, thermal analysis, and SEM, and all the results had corroborated for the chemical extraction of β-glucan polymers grafted amino groups by peptide bonds (namely NH2-β-glucan) as well as a cyclic oligosaccharide. Our primary purpose was to explore the polysaccharide extraction parameters in the sample of Usnea lichen, mainly in terms of extraction time and concentration of the extractive alkaline solution, to investigate the chemical characterization of the polysaccharide fractions. The low-cost methodology adopted in this work was able to extract the materials of interest with the expected efficiency without the significant impact of chemicals on the environment. The chemical extraction of an NH2-bonded biopolymer can be a very interesting material for many applications since chemical functionalization is crucial to introducing specific properties for developing new materials. In this regard, the cyclic β-glucan described as a very porous material is highly desirable for various applications in the biomedical, biotechnological, and environmental areas.



中文翻译:

鉴定松萝地衣中含酰胺的β-葡聚糖的方法学:光谱学研究

来自生物基质的聚合物馏分已通过振动光谱,XPS,CP / MAS 13 C NMR,MALDI-TOF质谱,热分析和SEM进行了充分表征 ,所有结果均已证实用于化学提取β-葡聚糖。聚合物通过肽键(即NH 2)接枝氨基-β-葡聚糖)和环状寡糖。我们的主要目的是主要从提取时间和碱性提取液的浓度方面研究松萝地衣样品中的多糖提取参数,以研究多糖级分的化学表征。在这项工作中采用的低成本方法能够以预期的效率提取感兴趣的材料,而不会对环境产生重大影响。NH 2的化学萃取键合的生物聚合物对于许多应用而言可能是非常有趣的材料,因为化学功能化对于引入开发新材料的特定特性至关重要。就这一点而言,被描述为非常多孔的材料的环状β-葡聚糖对于生物医学,生物技术和环境领域中的各种应用是高度期望的。

更新日期:2021-03-02
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