Plant-parasitic nematodes cause significant losses to coffee globally, but to date, there have been no reports of nematodes causing adverse effects to coffee crops in Australia. Four species of migratory endoparasitic nematodes, including Pratylenchus coffeae, Pratylenchus brachyurus, Pratylenchus zeae, and Radopholus similis, were detected in coffee-growing areas of Australia and their molecular data were also deposited in GenBank under accession numbers MN796428, MN718729, MN831891, and MN796426 respectively. A pathogenicity test of these nematode species was performed on seedlings of Coffea arabica cv. K7 under glasshouse conditions. We found that seedlings inoculated with P. coffeae and R. similis (both isolated from banana roots) and P. brachyurus (isolated from coffee roots), were stunted and their root systems were severely damaged. Tap roots and lateral roots of Coffea arabica cv. K7 were destroyed by P. coffeae, whereas P. brachyurus, and R. similis mainly damaged tap roots. Pratylenchus zeae, originally isolated from Saccharum spp., was not pathogenic to Coffea arabica cv. K7. This is the first report of nematode species capable of damaging coffee in Australia and the results demonstrate that nematodes pose a potential threat to coffee production.

植物寄生线虫在全球范围内对咖啡造成重大损失，但迄今为止，尚无线虫对澳大利亚咖啡作物造成不利影响的报道。在澳大利亚的咖啡种植区发现了四种迁徙性内生线虫，包括Prattylenchus coffeae，Prattylenchus brachyurus，Pratylenchus zeae和Radopholos similis，它们的分子数据也以登录号MN796428，MN718729，MN831891和MN796的形式保存在GenBank中。分别。在阿拉伯咖啡Cv的幼苗上进行了这些线虫物种的致病性测试。K7在温室条件下。我们发现接种了P. coffeae和R. similis的幼苗（均从香蕉根中分离）和短螺旋体（P. brachyurus）（均从咖啡根中分离）发育不良，其根系严重受损。阿拉比卡咖啡的自来根和侧根。K7被咖啡果破坏，而brachyurus和R. similis主要破坏水龙头根。短体线虫赤霉病，最初是从分离甘蔗属，是不致病的小粒种咖啡品种。K7。这是澳大利亚第一个能够破坏咖啡的线虫种类的报告，结果表明，线虫对咖啡生产构成了潜在的威胁。