ABSTRACT

Mauritia flexuosa L., traditionally known as “buriti”, exhibits chemoprotective properties including antioxidant, antithrombotic, and nutritional actions. The aim of this study was to examine the oral anti-inflammatory activity of epicarp, mesocarp and endocarp obtained from M. flexuosa fruits using in vivo models to verify physiological benefits. The anti-edematogenic action was determined using phlogistic agents to induce paw edema and peritonitis. Pro-inflammatory cytokines, cell migration of peritoneal cells, histological changes, and abdominal swelling induced by acetic acid were also investigated. Carrageenan-induced edema was found to be decreased in mice pre-treated with epicarp by 50.8%, 53.7% and 39.2% and mesocarp by 41.8%, 65.3% and 71.9% after 2, 3, and 4 hr stimuli, respectively. Edema initiated by specific agents such as compound 48/80, histamine, serotonin, and prostaglandin E₂ were also reduced, and better outcomes were found against histamine-induced edema, as evidenced by the decline at all times analyzed (30–120 min) with both doses of water extract of mesocarp (500 or 1000 mg/kg). Mesocarp-pre-treatment reduced inflammatory tissue parameters such as number of peritoneal leukocytes and TNF-α levels, but only epicarp diminished abdominal pain. In summary, M. flexuosa fruits, especially mesocarp, exhibited oral physiological benefits and capacity to modify biochemical and cellular steps in the inflammatory cascade, indicating that dietary supplements containing these fruits may be combined with pharmacological tools to ameliorate or prevent diseases of inflammatory origin.

摘要

Mauritia flexuosa L.，传统上称为“buriti”，具有化学保护特性，包括抗氧化、抗血栓形成和营养作用。本研究的目的是在体内检测从M. flexuosa果实中获得的外果皮、中果皮和内果皮的口服抗炎活性。验证生理益处的模型。抗水肿作用是通过使用炎药诱导爪水肿和腹膜炎来确定的。还研究了促炎细胞因子、腹膜细胞的细胞迁移、组织学变化和由乙酸引起的腹部肿胀。在 2、3 和 4 小时刺激后，用外果皮预处理的小鼠分别减少了 50.8%、53.7% 和 39.2% 和中果皮，分别减少了 41.8%、65.3% 和 71.9% 的角叉菜胶诱导的水肿。由特定药物引起的水肿，如化合物 48/80、组胺、血清素和前列腺素 E ₂也减少了，并且发现了更好的对抗组胺诱导的水肿的结果，正如使用两种剂量的中果皮水提取物（500 或 1000 毫克/千克）分析的所有时间（30-120 分钟）的下降所证明的那样。中果皮预处理降低了炎症组织参数，如腹膜白细胞数量和 TNF-α 水平，但只有外果皮减轻了腹痛。总之，M. flexuosa果实，尤其是中果皮，表现出口腔生理益处和改变炎症级联中生化和细胞步骤的能力，表明含有这些果实的膳食补充剂可以与药理学工具结合使用，以改善或预防炎症性疾病。