当前位置: X-MOL 学术Eur. J. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Mycotoxigenic fungi contaminating greenhouse-grown tomato fruit and their alternative control
European Journal of Plant Pathology ( IF 1.8 ) Pub Date : 2021-03-01 , DOI: 10.1007/s10658-021-02240-9
Simona Marianna Sanzani , Ferielle Djenane , Ornella Incerti , Naouel Admane , Annamaria Mincuzzi , Antonio Ippolito

Tomato fruit represent a valuable but perishable commodity due to postharvest fungal spoilage. In the present investigation, greenhouse-grown tomatoes were analysed, particularly for the contamination by mycotoxigenic fungi. Collected isolates were characterized by morphological features and sequencing of barcoding genes. Alternaria, Aspergillus, and Penicillium proved to be the most represented genera. Particularly, Alternaria strains were mostly A. alternata morphotype alternata, genetically able to produce alternariol and alternariol monomethyl ether. Aspergillus strains were divided into yellow and black aspergilli; the former included the species A. westerdijkiae, A. ochraceus, and A. occultus, able to produce ochratoxin A (OTA), the latter included an A. welwitschiae strain, genetically able to produce OTA and fumonisins. Most Penicillium strains were represented by P. olsonii, not able to produce regulated mycotoxins. Commercial Generally Recognized As Safe (GRAS) and biostimulant formulations were tested for controlling those pathogens and related decays. Potassium bicarbonate and the combination peracetic acid+hydrogen peroxide proved to completely prevent fungal growth. The same formulations were tested on tomato fruit against A. alternata morphotype alternata, which proved to be the most aggressive pathogen. Tests confirmed potassium bicarbonate and the combination peracetic acid+hydrogen peroxide as effective against disease incidence and severity, particularly when applied by dipping. These promising results support their use in larger-scale trials.



中文翻译:

致毒毒素真菌污染温室种植的番茄果实及其替代控制

由于收获后的真菌变质,番茄果实是一种有价值的但易腐烂的商品。在本次调查中,分析了温室种植的西红柿,特别是对于真菌毒素污染真菌的污染。收集的分离物的特征是条形码的形态特征和测序。交链孢菌曲霉青霉菌被证明是最有代表性的属。特别是,链格孢菌株大多A.孢形态型,基因能够产生交链孢和交链孢单甲醚。曲霉菌株分为黄色和黑色曲霉。前者包括A物种。westerdijkiae,曲霉和隐匿曲霉,能够产生曲霉毒素A(OTA),后者包括遗传上能够产生OTA和伏马菌素的welwitschiae菌株。大多数青霉菌株均以不能产生受调节的霉菌毒素为特征的P. olsonii为代表。测试了商业上公认的安全(GRAS)和生物刺激制剂,以控制那些病原体和相关的腐烂。碳酸氢钾和过氧乙酸+过氧化氢的组合被证明可以完全阻止真菌的生长。在番茄果实上测试了相同的配方以对抗链孢霉的形态型链孢菌,被证明是最具侵略性的病原体。测试证实了碳酸氢钾和过氧乙酸+过氧化氢的结合对疾病的发生和严重程度有效,特别是通过浸涂时。这些有希望的结果支持它们在大规模试验中的使用。

更新日期:2021-03-01
down
wechat
bug