Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as an endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET [TPLATE complex (TPC)] using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generate fresh insight into the differences between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.

真核细胞依靠内吞作用来调节它们的质膜蛋白质组和脂质组。除真菌和动物外，大多数真核生物群都保留了进化的古老 TSET 复合物作为内吞调节剂。与其他涂层聚合物复合物不同，缺乏对 TSET 的结构洞察力。在这里，我们使用整合结构方法揭示了植物 TSET [TPLATE complex (TPC)] 的分子结构。我们确定了特定 TSET 亚基在复杂组装和膜相互作用中的关键作用。因此，我们的数据对网柄菌中六聚体 TSET 之间的差异产生了新的见解和植物中的八聚体 TPC。这种古老的适配器复合物的结构阐明代表了涂层难题中缺失的部分，并极大地促进了我们对内吞过程的功能和进化洞察力。