Bacillus megaterium strain BHS1, isolated from an alkaline water sample taken from Mavi Gölü (Blue Lake, Turkey), can grow on minimal medium containing 2,2-dichloropropionic acid. We characterized this bacterium at the genomic level. The HiSeq platform was used to carry out genome sequencing, de novo assembly, and scaffolding with strain BHS1. Next, genome data were analyzed to demarcate DNA regions containing protein-coding genes and determine the function of certain BHS1 genes. Finally, results from a colorimetric chloride ion–release assay demonstrated that strain BHS1 produces dehalogenase. De novo assembly of the BHS1 genomic sequence revealed a genome size of ~ 5.37 Mb with an average G+C content of 38%. The predicted nuclear genome harbors 5509 protein-coding genes, 1353 tRNA genes, 67 rRNA genes, and 6 non-coding (mRNA) genes. Genomic mapping of strain BHS1 revealed its amenability to synthesize two families of dehalogenases (Cof-type haloacid dehalogenase IIB family hydrolase and haloacid dehalogenase type II), suggesting that these enzymes can participate in the catabolism of halogenated organic acids. The mapping identified seven Na+/H+ antiporter subunits that are vital for adaptation of the bacterium to an alkaline environment. Apart from a pairwise analysis to the well-established L-2-haloacid dehalogenases, whole-cell analysis strongly suggested that the haloacid dehalogenase type II might act stereospecifically on L-2-chloropropionic acid, D,L-2-chloropropionic acid, and 2,2-dichloropropionic acid. Whole-cell studies confirmed the utilization of these three substrates and the gene’s role in dehalogenation. To our knowledge, this is the first report of the full genome sequence for strain BHS1, which enabled the characterization of selected genes having specific metabolic activities and their roles in the biodegradation of halogenated compounds.

中文翻译：

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从蓝湖分离的巨大芽孢杆菌BHS1菌株的功能性卤酸降解基因的基因组分析（土耳其玛维·格吕）

从取自MaviGölü（土耳其蓝湖）的碱性水样品中分离出的巨大芽孢杆菌BHS1菌株可以在含有2，2-二氯丙酸的基本培养基上生长。我们在基因组水平上表征了这种细菌。使用HiSeq平台进行基因组测序，从头组装和BHS1菌株支架。接下来，分析基因组数据以划定包含蛋白质编码基因的DNA区域，并确定某些BHS1基因的功能。最后，比色氯离子释放测定的结果表明，菌株BHS1产生脱卤酶。BHS1基因组序列的从头组装显示出〜5.37 Mb的基因组大小，平均G + C含量为38％。预测的核基因组包含5509个蛋白质编码基因，1353个tRNA基因，67个rRNA基因和6个非编码（mRNA）基因。菌株BHS1的基因组作图表明其能够合成两个脱卤酶家族（Cof型卤酸脱卤酶IIB家族水解酶和II型卤酸脱卤酶），表明这些酶可以参与卤代有机酸的分解代谢。该作图确定了七个Na + / H +反转运蛋白亚基，这些亚基对于细菌适应碱性环境至关重要。除了对成熟的L-2-卤代酸脱卤酶进行成对分析外，全细胞分析还强烈提示II型卤代酸脱卤酶可能对L-2-氯丙酸，D，L-2-氯丙酸和2,2-二氯丙酸。全细胞研究证实了这三种底物的利用以及该基因在脱卤中的作用。据我们所知，