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Integrated analysis of gait parameters and gene expression profiles in a murine model of subarachnoid hemorrhage
Genes, Brain and Behavior ( IF 2.5 ) Pub Date : 2021-02-28 , DOI: 10.1111/gbb.12728
Zhi Yuan Zheng 1 , Gang Lu 2 , Zhi Qiang Xiong 3 , Chi Kwan Leung 2 , Xian Wei Su 2 , Tu Li 2 , Wai Sang Poon 1 , Wai Yee Chan 2 , George Kwok Chu Wong 1
Affiliation  

Gait analysis has been widely used to examine the behavioral presentation of numerous neurological disorders. Thorough murine model evaluation of the subarachnoid hemorrhage (SAH)-associated gait deficits is missing. This study measures gait deficits using a clinically relevant murine model of SAH to examine associations between gait variability and SAH-associated gene expressions. A total of 159 dynamic and static gait parameters from the endovascular perforation murine model for simulating clinical human SAH were determined using the CatWalk system. Eighty gait parameters and the mRNA expression levels of 35 of the 88 SAH-associated genes were differentially regulated in the diseased models. Totals of 42 and 38 gait parameters correlated with the 35 SAH-associated genes positively and negatively with Pearson's correlation coefficients of >0.7 and <−0.7, respectively. p-SP1453 expression in the motor cortex in SAH animal models displays a significant correlation with a subset of gait parameters associated with muscular strength and coordination of limb movements. Our data highlights a strong correlation between gait variability and SAH-associated gene expression. p-SP1453 expression could act as a biomarker to monitor SAH pathological development and a therapeutic target for SAH.

中文翻译:

蛛网膜下腔出血小鼠模型中步态参数和基因表达谱的综合分析

步态分析已被广泛用于检查许多神经系统疾病的行为表现。缺乏对蛛网膜下腔出血 (SAH) 相关步态缺陷的全面鼠模型评估。本研究使用临床相关的 SAH 鼠模型测量步态缺陷,以检查步态变异性与 SAH 相关基因表达之间的关联。使用 CatWalk 系统确定了来自血管内穿孔小鼠模型的总共 159 个动态和静态步态参数,用于模拟临床人类 SAH。80 个步态参数和 88 个 SAH 相关基因中的 35 个的 mRNA 表达水平在患病模型中受到差异调节。总共 42 和 38 个步态参数与 35 个 SAH 相关基因正相关和负相关,皮尔逊相关系数 > 分别为 0.7 和 <-0.7。p-SP1SAH 动物模型中运动皮层中的453表达显示出与与肌肉力量和肢体运动协调相关的步态参数子集显着相关。我们的数据强调了步态变异性与 SAH 相关基因表达之间的强相关性。p-SP1 453表达可作为监测 SAH 病理发展的生物标志物和 SAH 的治疗靶点。
更新日期:2021-02-28
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