Here, an asymmetric double-layer membrane has been designed and fabricated by electrospinning as a tool for a potential wound healing application. A hydrophobic layer has been produced by using a polyurethane-polycaprolactone (PU-PCL) copolymer and loaded with the antibacterial ciprofloxacin whereas an ion responsive hydrophilic layer has been produced by using an octyl derivative of gellan gum (GG-C₈) and polyvinyl alcohol (PVA) and loaded with the growth factor FGF-2. This study investigated how the properties of this asymmetric membrane loaded with actives, were influenced by the ionotropic crosslinking of the hydrophilic layer. In particular, the treatment in DPBS and the crosslinking in CaCl₂ 0.1 or 1 M of the hydrophilic layer affected the release profile of the bioactive molecules allowing to modulate both the antimicrobial effect, as assayed by logarithmic reduction of the Staphylococcus aureus viable count, and the chemoattractant properties on NIH 3 T3 cell line, as assayed by scratch test and cell chemoattraction assay.

在此，已经通过电纺丝设计和制造了不对称的双层膜，作为潜在的伤口愈合应用的工具。通过使用聚氨酯-聚己内酯（PU-PCL）共聚物生产疏水层并负载抗菌环丙沙星，而通过使用吉兰糖胶（GG-C ₈）的辛基衍生物和聚乙烯醇生产离子响应性亲水层（PVA），并加入生长因子FGF-2。这项研究调查了这种负载活性物质的不对称膜的性能如何受到亲水层离子交联的影响。特别地，在DPBS中的处理和在CaCl _2中的交联亲水层的0.1或1 M影响了生物活性分子的释放曲线，从而既可以调节抗微生物作用（通过对数减少金黄色葡萄球菌存活数来测定），又可以通过NIH 3 T3细胞系对化学吸引力进行调节刮擦试验和细胞趋化试验。