Purpose

Grass pea is a legume species with recognized resistance to several diseases and thus important for the improvement of related major legume crops. It is infected by the soil-borne fungus Fusarium oxysporum f. sp. pisi (Fop), known as causal agent of pea fusarium wilt. We aim to identify, among grass pea, new sources of resistance against Fop and characterize the detected resistance mechanisms.

Methods

A grass pea worldwide collection of accessions was characterized under controlled conditions for response to Fop race 2. Fungal colonization dynamics and potential resistance mechanisms were studied through confocal laser scanning microscopy (CLSM) using Fop race 2 expressing green fluorescent protein (GFP).

Results

A quantitative nature of resistance to Fop, ranging from highly to partially resistant and susceptible accessions was detected, with resistance being the most frequent phenotype. Diverse colonization patterns were observed, suggesting the existence of different resistance mechanisms. In the highly resistant accessions, absence of fungal colonization in the vascular tissue was detected, while fungal progression was arrested at the level of roots both in highly resistant and partially resistant accessions.

Conclusions

The resistant accessions identified here can be exploited in grass pea breeding for fusarium wilt resistance, and due to the phylogenetic relatedness to pea, potentially contribute to pea improvement.

中文翻译：

---

尖孢镰刀菌f。sp。草豌豆种质中发现的pisi

目的

豌豆是一种豆科植物，对多种疾病都有抵抗力，因此对于改善相关的主要豆类作物很重要。它被土壤传播的真菌尖孢镰刀菌（Fusarium oxysporum） f感染。sp。豌豆（Fop），被称为豌豆枯萎病的病因。我们的目标是在豌豆中识别出对Fop的新抗性来源，并描述检测到的抗性机制。

方法

在可控制的条件下对Fop race 2进行响应的条件下，对全球范围内的豌豆种质进行了表征，使用表达绿色荧光蛋白（GFP）的Fop race 2通过共聚焦激光扫描显微镜（CLSM）研究了真菌定植动力学和潜在的抗性机制。

结果

检测到对Fop的抗性的定量性质，范围从高到部分抗性和易感种质，其中抗性是最常见的表型。观察到不同的定殖模式，表明存在不同的抗性机制。在高抗性种质中，在血管组织中未检测到真菌定植，而在高抗性和部分抗性种质中，真菌的生长都在根部被阻止。

结论

此处鉴定出的抗性材料可以在豌豆育种中用于枯萎病的抗性，并且由于与豌豆的系统发育相关性，可能有助于豌豆的改良。