The objective of the present study was to evaluate the action of benzocaine and eugenol over anesthesia stages and the use of three anticoagulants (ethylenediamine tetraacetic acid, sodium citrate, and sodium heparin) over hematological and biochemical parameters and erythrocyte osmotic fragility in tambaquis Colossoma macropomum. For that, fish were distributed into two experimental groups: G1: fish anesthetized with benzocaine and G2: fish anesthetized with eugenol. Induction and recovery times of anesthesia were evaluated and blood samples with different anticoagulants were collected for hematological and biochemical analysis and osmotic erythrocyte osmotic fragility. Fish anesthetized with eugenol showed a longer period of anesthesia, shorter induction time, and longer recovery time. In addition, fish anesthetized with benzocaine by using sodium citrate showed leukopenia and lymphopenia and morphological alterations when compared to the other anticoagulants. Furthermore, fish anesthetized with eugenol by using sodium citrate showed significantly lower values (P < 0.05) of hematocrit and hemoglobin when compared to other anticoagulants and there were significant differences in erythrocyte osmotic fragility between anticoagulants in fish anesthetized with eugenol. The results observed in this study indicate that eugenol is a good alternative as anesthetics for tambaquis when compared to benzocaine because of its rapid anesthesia induction and few alterations in blood parameters. In addition, the use of EDTA facilitates the erythrocyte conservation and cause few blood alterations compared to other anticoagulants.

本研究的目的是评估苯巴卡因和丁香酚在麻醉阶段的作用，以及三种抗凝剂（乙二胺四乙酸，柠檬酸钠和肝素钠）对血常规和生化指标以及红血球渗透性脆弱性的使用。为此，将鱼分为两个实验组：G1：用苯佐卡因麻醉的鱼和G2：丁香酚麻醉的鱼。评价麻醉的诱导和恢复时间，并收集具有不同抗凝剂的血样进行血液学和生化分析以及渗透性红细胞渗透性脆性。用丁香酚麻醉的鱼显示出更长的麻醉时间，更短的诱导时间和更长的恢复时间。此外，与其他抗凝剂相比，用柠檬酸钠苯佐卡因麻醉的鱼显示出白细胞减少和淋巴细胞减少以及形态改变。此外，与其他抗凝剂相比，丁香酚柠檬酸钠麻醉的鱼的血细胞比容和血红蛋白值显着较低（P <0.05），并且红细胞渗透性脆弱性也存在显着差异用丁子香酚麻醉的鱼中的抗凝剂之间的关系 在这项研究中观察到的结果表明，与苯并卡因相比，丁香酚是一种很好的替代坦巴奎的麻醉剂，因为它可以快速诱导麻醉并且血液参数几乎没有变化。此外，与其他抗凝剂相比，EDTA的使用促进了红细胞的保存并且几乎没有引起血液改变。