Reactive oxygen and nitrogen species have been implicated in the onset and progression of various diseases and the role of antioxidants in the maintenance of health and prevention of diseases has received much attention. The action and effect of antioxidants have been studied extensively under different reaction conditions in multiple media. The antioxidant effects are determined by many factors. This review aims to discuss several important issues that should be considered for determination of experimental conditions and interpretation of experimental results in order to understand the beneficial effects and limit of antioxidants against detrimental oxidation of biological molecules. Emphasis was laid on cell culture experiments and effects of diversity of multiple oxidants on antioxidant efficacy.

中文翻译：

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影响体外和体内抗氧化作用的因素。实验条件和氧化剂的性质决定抗氧化剂的功效

活性氧和氮物质与各种疾病的发作和发展有关，抗氧化剂在维持健康和预防疾病中的作用已引起广泛关注。抗氧化剂的作用和效果已在多种介质中不同反应条件下进行了广泛研究。抗氧化剂作用由许多因素决定。这篇综述旨在讨论确定实验条件和解释实验结果时应考虑的几个重要问题，以了解抗氧化剂对生物分子有害氧化的有益作用和局限性。重点放在细胞培养实验上，以及多种氧化剂的多样性对抗氧化剂功效的影响。