We present a modular implementation of structured illumination microscopy (SIM) that is fast, largely self-contained and that can be added onto existing fluorescence microscopes. Our instrument, which we call HIT-SIM, can theoretically deliver well over 50 super-resolved images per second and is readily compatible with existing acquisition software packages. We provide a full technical package consisting of schematics, a list of components and an alignment scheme that provides detailed specifications and assembly instructions. We illustrate the performance of the instrument by imaging optically large samples containing sequence-specifically stained DNA fragments.

中文翻译：

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自成一体的模块化结构照明显微镜

我们提出了一种结构化照明显微镜（SIM）的模块化实现，该模块实现了快速，很大程度上独立的功能，可以添加到现有的荧光显微镜中。从理论上讲，我们的仪器称为HIT-SIM，每秒可以提供50幅以上的超分辨率图像，并且可以轻松兼容现有的采集软件包。我们提供完整的技术包，包括原理图，组件列表和对齐方案，其中提供详细的规格和组装说明。我们通过对包含序列特异性染色的DNA片段的光学大样品进行成像来说明仪器的性能。