We describe a multimodal SCATTIRSTORM microscope for visualizing processive enzymes moving on immobilized substrates. The instrument combines Interference Reflection Microscopy (IRM) with multi-wavelength Total Internal Reflectance Fluorescence Microscopy (TIRFM). The microscope can localize quantum dots with a precision of 2.8 nm at 100 frames/s, and was used to image the dynamics of the cellulase, Cel7a interacting surface-immobilized cellulose. The instrument, which was built with off-the-shelf components and controlled by custom software, is suitable for tracking other degradative enzymes such as collagenases, as well as motor proteins moving along immobilized tracks.

中文翻译：

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集成了TIRFM和IRM模态的集成多波长SCATTIRSTORM显微镜，可对纤维素酶和其他过程性酶进行成像

我们描述了一种多模式的SCATTIRSTORM显微镜，用于可视化在固定化底物上移动的过程性酶。该仪器将干涉反射显微镜（IRM）与多波长全内反射荧光显微镜（TIRFM）结合在一起。显微镜可以100帧/秒的速度精确定位2.8 nm的量子点，并用于对纤维素酶Cel7a相互作用的表面固定化纤维素的动力学进行成像。该仪器由现成的组件构成，并由定制软件控制，适用于跟踪其他降解酶，例如胶原酶，以及沿着固定轨道移动的运动蛋白。