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Faecal bacterial composition in horses with and without free faecal liquid: a case control study
Scientific Reports ( IF 4.6 ) Pub Date : 2021-02-26 , DOI: 10.1038/s41598-021-83897-4
Katrin M Lindroth 1 , Johan Dicksved 1 , Erik Pelve 2 , Viveca Båverud 3 , Cecilia E Müller 1
Affiliation  

Free faecal liquid (FFL) is a condition in horses which manifests as differential defecation of solid and liquid phases of faeces. The etiology of FFL is currently unknown, but deviances in the hindgut microbiota has been suggested to be of importance. The present study aimed to compare the faecal bacterial composition of farm-matched horses with (case, n = 50) and without (control, n = 50) FFL. Samples were collected at three different occasions. The V3 and V4 regions of the 16S rRNA gene were amplified and sequenced using Illumina sequencing. Also, samples were cultivated for detection of Clostridioides difficile and Clostridium perfringens. Analysis revealed similar faecal bacterial composition between case and control horses, but an effect of sampling period (p = 0.0001). Within sampling periods, 14 genera were present in higher or lower proportions in case compared to control horses in at least one sampling period. Compared to controls, case horses had higher relative abundance of Alloprevotella (adjusted p < 0.04) and lower relative abundance of Bacillus spp. (adjusted p < 0.03) in at least two sampling periods. All horses tested negative for C. difficile and C. perfringens by culture of faeces. Further studies are required to establish the clinical relevance of specific bacterial taxa in FFL.



中文翻译:

有和没有游离粪便液的马的粪便细菌组成:病例对照研究

游离粪便液体 (FFL) 是马的一种病症,表现为粪便固相和液相的排便不同。FFL 的病因目前尚不清楚,但已表明后肠微生物群的异常具有重要意义。本研究旨在比较农场匹配马匹(病例,n = 50)和没有(对照,n = 50)FFL 的粪便细菌组成。在三个不同的场合收集样品。使用Illumina测序对16S rRNA基因的V3和V4区域进行扩增和测序。此外,培养样品以检测艰难梭菌产气荚膜梭菌. 分析显示病例马和对照马之间的粪便细菌组成相似,但受采样周期的影响(p = 0.0001)。在采样周期内,至少在一个采样周期内,与对照马相比,有 14 个属以较高或较低的比例存在。与对照相比,病例马的Alloprevotella相对丰度更高(调整后的 p < 0.04),而芽孢杆菌属的相对丰度更低(调整后的 p < 0.03)至少在两个采样周期内。通过粪便培养,所有马的艰难梭菌产气荚膜梭菌检测均为阴性。需要进一步的研究来确定 FFL 中特定细菌分类群的临床相关性。

更新日期:2021-02-26
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