当前位置: X-MOL 学术J. Hazard. Mater. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Highly stable selenium nanoparticles: Assembly and stabilization via flagellin FliC and porin OmpF in Rahnella aquatilis HX2
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2021-02-26 , DOI: 10.1016/j.jhazmat.2021.125545
Kui Li , Qiaolin Xu , Shanshan Gao , Sasa Zhang , Yuhui Ma , Guishen Zhao , Yanbin Guo

Microorganisms play a critical role in the reduction of the more toxic selenite and selenate to the less toxic elemental selenium. However, the assembly process and stability of selenium nanoparticles (SeNPs) remain understudied. The plant growth-promoting rhizobacterium Rahnella aquatilis HX2 can reduce selenite to biogenic SeNPs (BioSeNPs). Two main proteins, namely flagellin FliC and porin OmpF were identified in the BioSeNPs. The fliC and ompF gene mutation experiments demonstrated that the FliC and OmpF could control the assembly of BioSeNPs in vivo. At the same time, the expressed and purified FliC and OmpF could control the assembly of SeNPs in vitro. BioSeNPs produced by R. aquatilis HX2 exhibited high stability under various ionic strengths, while the chemically synthesized SeNPs (CheSeNPs) showed a high level of aggregation. The in vitro experiments verified that FliC and OmpF could prevent the aggregation of the CheSeNPs under various ionic strengths. This work reports the preparation of highly stable BioSeNPs produced by strain R. aquatilis HX2 and verifies that FliC and OmpF both could control the assembly and stability of BioSeNPs. BioSeNPs with high stability could be suitable as nutritional supplement to remedy selenium deficiency and in nanomedicine applications.



中文翻译:

高度稳定的硒纳米颗粒:通过鞭毛蛋白FliC和孔蛋白OmpF在水生拉氏菌HX2中进行组装和稳定化

微生物在减少毒性更大的亚硒酸盐并硒化为毒性较小的元素硒中起关键作用。然而,硒纳米粒子(SeNPs)的组装过程和稳定性仍未得到充分研究。的植物生长促进根际细菌水生拉恩HX2可以减少亚硒酸钠到生物SeNPs(BioSeNPs)。在BioSeNPs中鉴定了两种主要蛋白质,即鞭毛蛋白FliC和孔蛋白OmpF。所述的fliCOmpF的基因突变实验表明,FLIC和OmpF的可以控制BioSeNPs的装配体内。同时,表达和纯化的FliC和OmpF可以在体外控制SeNP的装配。BioSeNPs产生由R. aquatilisHX2在各种离子强度下均显示出高稳定性,而化学合成的SeNPs(CheSeNPs)显示出高水平的聚集。在体外实验证实,FLIC和OmpF的可以防止在各种离子强度的CheSeNPs的聚集。这项工作报告了由水生R. aquatilis HX2菌株产生的高度稳定的BioSeNPs的制备,并验证了FliC和OmpF都可以控制BioSeNPs的组装和稳定性。具有高稳定性的BioSeNPs可能适合作为营养补品,用于治疗硒缺乏症和纳米医学应用。

更新日期:2021-03-03
down
wechat
bug