In this study, we developed a novel dual-analytical platform for the visual, sensitive, and reliable analysis of mercury ions (Hg²⁺) in environmental water samples. Importantly, thymine (T)-rich DNA probes were utilized to form T-Hg²⁺-T base pairs in the presence of Hg²⁺ to ensure the specificity of the method. We synthesized new luminescent tris(4,4′-dicarboxylicacid-2,2′-bipyridyl) ruthenium (II) dichloride (Ru(dcbpy)₃²⁺)-modified metal-polydopamine frameworks (MPFs@Ru), which were then applied to construct an electrochemiluminescence (ECL) system for the first time, and it achieved accurate and sensitive quantitative detection of Hg²⁺. To achieve rapid on-site determination, a multicolorimetric system based on a smartphone was established by inducing deposition of silver shells on gold nanorods (Au NRs). Under optimized conditions, the dual-modal assay showed an excellent response for Hg²⁺ in the linear range of 2 pmol L⁻¹ to 500 nmol L⁻¹, with a low detection limit of 0.32 pmol L⁻¹. Moreover, the proposed method demonstrated satisfactory selectivity, stability, and acceptable reproducibility for the detection of Hg²⁺. The recovery of lake water samples ranged from 98.53% to 111.97% for the ECL method and from 95.04% to 106.11% for the colorimetric method, indicating the potential applicability of the proposed method for monitoring environmental water samples.

在这项研究中，我们开发了一种新颖的双重分析平台，用于可视，灵敏和可靠地分析环境水样品中的汞离子（Hg ²⁺）。重要的是，在Hg ²⁺存在下，利用富含胸腺嘧啶（T）的DNA探针形成T-Hg ²⁺ -T碱基对，以确保该方法的特异性。我们合成了新的发光的三（4,4'-二羧酸-2,2'-联吡啶）钌（II）二氯化钌（Ru（dcbpy）_{3 2} ⁺）-修饰的金属-聚多巴胺骨架（MPFs @ Ru），然后将其应用首次构建电化学发光（ECL）系统，并实现了准确，灵敏的Hg ²⁺定量检测。为了实现快速现场确定，通过诱导银壳在金纳米棒（Au NRs）上的沉积，建立了基于智能手机的多色系统。在优化条件下，双峰分析显示Hg ²⁺在2 pmol L ^-1至500 nmol L ^-1的线性范围内具有出色的响应，检出限低至0.32 pmol L ^-1。此外，该方法对Hg ²⁺的检测具有令人满意的选择性，稳定性和可接受的重现性。。ECL方法的湖水样品回收率从98.53％到111.97％，比色法的湖水样品回收率从95.04％到106.11％，表明该方法在监测环境水样中的潜在适用性。