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CDX-2 Protein and mRNA Expression in Canine Intestinal Adenocarcinoma
Journal of Comparative Pathology ( IF 0.8 ) Pub Date : 2021-02-26 , DOI: 10.1016/j.jcpa.2021.01.010
Seung-Hee Cho 1 , Byung-Joon Seung 1 , Soo-Hyeon Kim 1 , Min-Kyung Bae 1 , Ha-Young Lim 1 , Jung-Hyang Sur 1
Affiliation  

Caudal-related homeobox transcription factor 2 (CDX-2) is a specific cell marker employed in the diagnosis of human colorectal cancer. Reduced CDX-2 expression is associated with several indicators of poor prognosis in human colorectal cancer. In the present study, CDX-2 protein levels were evaluated and patterns of CDX-2 mRNA accumulation are described for the first time in canine intestinal adenocarcinoma (CIA). Canine intestinal epithelial biopsies from 21 CIAs and 14 non-neoplastic control tissues were retrospectively evaluated for CDX-2 expression and CDX-2 mRNA levels by immunohistochemistry and RNA in-situ hybridization (RNA-ISH), respectively. The mean percentage or intensity of expression was decreased in the CIA group (P = 0.000). RNA-ISH demonstrated a significant correlation between the decrease in CDX-2 mRNA levels and CDX-2 protein expression (P = 0.000). CDX-2 downregulation, in terms of protein as well as mRNA levels, may serve as a diagnostic marker in CIA.



中文翻译:

CDX-2蛋白和mRNA在犬肠腺癌中的表达

尾端相关同源盒转录因子 2 ( CDX-2 ) 是一种用于诊断人类结直肠癌的特异性细胞标志物。CDX-2表达降低与人类结直肠癌预后不良的几个指标有关。在本研究中,评估了CDX-2蛋白水平,并首次描述了犬肠腺癌 (CIA)中CDX- 2 mRNA 积累的模式。回顾性评估了来自 21 个 CIA 和 14 个非肿瘤对照组织的犬肠上皮活检的 CDX-2 表达和CDX-2 mRNA 水平分别通过免疫组织化学和 RNA 原位杂交 (RNA-ISH)。CIA 组的平均表达百分比或强度降低(P  = 0.000)。RNA-ISH证明在减少之间的相关性显著CDX- 2 mRNA水平和CDX-2蛋白的表达(P  = 0.000)。CDX-2下调,在蛋白质和 mRNA 水平方面,可作为 CIA 的诊断标志物。

更新日期:2021-02-26
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