Anaerobic digestion is a possibility for post-hydrothermal liquefaction wastewater (PHWW) treatment because this wastewater is rich in nutrients and organic compounds. However, the PHWW presents many toxic compounds. A strategy for the anaerobic treatment of toxic compounds is using biomass adhered to inert supports forming biofilms, which can offer more resistance to the microorganism and protection from such compounds. The continuous treatment of PHWW is the essential key to obtaining a sustainable hydrothermal liquefaction process. In this work, the use of immobilized biomass was evaluated for the anaerobic degradation of PHWW from Spirulina in batch assays and continuous treatment. Higher methane production potential and volatile fatty acid mass balance showed the advantages of using biomass immobilized in polyurethane foam. Continuous treatment in a horizontal-flow anaerobic immobilized biomass (HAIB) reactor reached chemical organic demand (COD) removal efficiencies of 67% and 58% for volumetric organic load rates of 0.8 and 1.6 g COD.L⁻¹.d⁻¹, respectively. After 200 days of continuous treatment, Anaerobaculum and Coprothermobacter, fermentative proteolytic genera of bacteria with potential for hydrogen production, were favored.

厌氧消化是水热液化后废水（PHWW）处理的一种可能性，因为该废水中富含营养成分和有机化合物。但是，PHWW存在许多有毒化合物。毒性化合物的厌氧处理策略是使用粘附到形成生物膜的惰性载体上的生物质，该生物膜可提供对微生物的更大抵抗力并免受此类化合物的侵害。PHWW的连续处理是获得可持续的水热液化过程的关键。在这项工作中，使用固定化的生物质评估了螺旋藻对PHWW的厌氧降解。分批测定和连续治疗。较高的甲烷生产潜力和挥发性脂肪酸质量平衡显示了使用固定在聚氨酯泡沫塑料中的生物质的优势。在水平流厌氧固定化生物质（HAIB）反应器中进行连续处理，有机物的容积率分别为0.8和1.6 g COD.L ^-1 .d ^-1时，化学有机需求（COD）去除效率分别为67％和58％。。经过200天的连续处理后，厌氧菌和Coprothermobacter细菌（具有产氢潜力的细菌的发酵蛋白水解属）受到青睐。