A total of 50 Escherichia coli were isolated from buffalo meat and their antibiotic profiling was carried out. 90% E. coli isolates showed resistant to two or more classes of 21 commonly used antibiotics. Moreover, there was also variation in resistance/sensitivity behavior towards antibiotics. Highest resistance was found to be against methicillin (84%) in the isolates followed by vancomicin (70%), sulphadiazine (68%) and cefaclor (66%), whereas, resistance was less common for kanamycin (8%) and chloramphenicol (4%). ECMB1, ECMA2, ECMA8, ECMS9 and ECMA31 strains showed highest MDR pattern with presence of ^blaCTX-M, qnr S and qnr B resistant genes. ECMB1 strain was resistant to 14 antibiotics belonging to 7 different classes. Therefore, ECMB1 was selected for further studies. Sodium Alginate Film incorporated with 10, 20, and 30% ethanolic extract of Syzygium cumini (EESC) were formulated and characterized using state-of-art techniques. A dose-dependent antibacterial activity against E. coli ECMB1 was recorded by the films made from EESC (EESCF). The growth kinetics of E. coli strain ECMB1 showed 9% decrease in log CFU when it was cultured in 30% EESCF as compared to control cells after 12 h of growth. Present finding highlight the efficacy and possible use of EESCF as meat packaging film to prevent food spoilage caused by MDR bacteria.

共从水牛肉中分离出 50株大肠杆菌，并对其进行了抗生素分析。90%的大肠杆菌分离株对两类或更多类 21 种常用抗生素具有抗药性。此外，对抗生素的抗性/敏感性行为也存在差异。在分离物中发现对甲氧西林的耐药性最高（84%），其次是万古霉素（70%）、磺胺嘧啶（68%）和头孢克洛（66%），而对卡那霉素（8%）和氯霉素的耐药性较低。 4%）。ECMB1、ECMA2、ECMA8、ECMA9 和 ECMA31 菌株显示出最高的 MDR 模式，其中存在^bla CTX-M、qnr S和qnr B抗性基因。ECMB1菌株对属于7个不同类别的14种抗生素具有抗性。因此，选择ECMB1进行进一步研究。使用最先进的技术配制和表征掺有 10、20 和 30%蒲桃乙醇提取物 ( EESC) 的海藻酸钠薄膜。由 EESC (EESCF) 制成的薄膜记录了对大肠杆菌ECMB1的剂量依赖性抗菌活性。与生长12 小时后的对照细胞相比，在 30% EESCF 中培养时，大肠杆菌菌株 ECMB1的生长动力学显示 log CFU 减少 9%。目前的发现突出了 EESCF 作为肉类包装薄膜的功效和可能的用途，以防止由 MDR 细菌引起的食品变质。