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Effect of horseshoe crab perivitelline fluid on the viability of stem cells from human exfoliated deciduous teeth and expression of cell cycle regulatory genes
Indian Journal of Geo-Marine Sciences ( IF 0.5 ) Pub Date : 2021-02-25
I Najian, T P Kannan, A Azlina, I M Khairani

The effect of perivitelline fluid (PVF) (0.019 mg/ml) on the stem cell viability extracted from human exfoliated deciduous teeth was assessed using LIVE/DEAD viability/cytotoxicity. It showed that PVF enhanced viability as observed by higher live cell percentage and a greater number of viable cells in the treated group for 3 days. The expression of selected cell cycle regulatory genes investigated using reverse transcriptase polymerase chain reaction (RT-PCR) for 21 days showed that CDKN2A, PTEN and TP53 expressed significantly higher in the treated group, suggesting that PVF enhanced SHED growth and proliferation. MDM2 expression remained at low levels in the treated group indicating that PVF did not result in tumorigenic growth. Low expression of apoptotic activator gene, BCL2L11 in treated group from day 1 until 14 with a sudden peak on day 21 was noted. This is deemed normal as the cells thrive to maintain homeostatic level at the 21st day of incubation.

中文翻译:

crab螃蟹卵白质液对人脱落乳牙干细胞活力及细胞周期调控基因表达的影响

使用LIVE / DEAD生存力/细胞毒性评估了玻璃体周液(PVF)(0.019 mg / ml)对从人脱落的乳牙中提取的干细胞生存力的影响。结果表明,治疗3天后,通过提高活细胞百分比和增加活细胞数量,PVF增强了活力。使用逆转录聚合酶链反应(RT-PCR)研究21天的选定细胞周期调控基因的表达表明,CDKN2A,PTENTP53在治疗组中表达明显更高,表明PVF增强了SHED的生长和增殖。MDM2表达在治疗组中保持在低水平,表明PVF没有导致致瘤性生长。从第1天到第14天,治疗组中凋亡激活基因BCL2L11的表达低,在第21天突然达到峰值。作为细胞茁壮成长维持在21级稳态这被认为是正常的ST孵化的一天。
更新日期:2021-02-25
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