Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.

中文翻译：

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杨树中羟肉桂酰辅酶A：sh草酸羟肉桂酰转移酶（HCT）基因家族的特征与功能分析

羟肉桂酰辅酶A：sh草酸酯羟肉桂酰转移酶（HCT）将质量通量分为单木质素生物合成中的H，G和S单位，并影响木质素含量。在毛果杨（Torr。＆Gray）基因组中鉴定出十个HCT同源物。基因组重复和串联重复均导致胡杨中HCT直系同源物的扩增。包括基序分析，系统发育分析，表达谱和共表达分析在内的综合分析揭示了这些候选PoptrHCT的差异性和推定功能。PoptrHCT1和2被确定可能参与了木质素的生物合成。PoptrHCT9和10-可能参与植物发育以及对冷胁迫的反应。在毛白杨中也发现了类似的功能差异。