For a long time, it has been known that seed priming allows the improvement of plant production and tolerance to abiotic stresses. However, a negative effect on the longevity of the seeds thus primed was observed; these mechanisms are still poorly understood. In addition, it has been shown by several authors that seed ageing is associated with the oxidation and particularly with carbonylation of protein. Our work consisted in studying the AOPP and carbonyl protein at the different parts of the embryo from freshly primed seeds and from those that have been primed for 4 years (after storage). We subjected Vigna unguiculata (L.) Walp. seeds to a single or double hydropriming. Our study showed that hydropriming, and more particularly a double cycle of hydration-dehydration, makes it possible to attenuate the oxidation of the protein while it favours a certain threshold of carbonylation in the freshly dehydrated seeds in order to better trigger the germination process. On the other hand, after a storage period of 4 years, these dehydrated seeds are characterised by a strong accumulation of the products of oxidation and especially carbonylated protein, compared with the untreated seeds, which could explain the decrease of the longevity of these seeds.

长期以来，已知种子引发可以改善植物的产量和对非生物胁迫的耐受性。然而，观察到这样引发的种子的寿命受到负面影响；这些机制仍然知之甚少。另外，一些作者已经表明，种子老化与蛋白质的氧化特别是羰基化有关。我们的工作包括研究来自刚启动的种子和已经启动4年（储存后）的种子在胚胎不同部位的AOPP和羰基蛋白。我们对Vigna unguiculata进行了处理（L.）Walp。种子进行单次或两次加水处理。我们的研究表明，加水引发，尤其是水合-脱水的双循环，可以减弱蛋白质的氧化，同时有利于新鲜脱水种子中的羰基化一定阈值，从而更好地触发发芽过程。另一方面，与未处理的种子相比，经过4年的保存期，这些脱水种子的特征在于氧化产物，尤其是羰基化蛋白质的强积聚，这可以解释这些种子的寿命降低。