Fluoride has been found to cause detrimental effects on fish gills. Despite essential roles in various metabolism activities, whether and how miRNAs participate in the course of toxicity caused by fluoride in gills is still unclear. In this study, male zebrafish were exposed to 0, 20, 40 mg/L fluoride for 60 days to study the underlying osmotic regulatory mechanism by determining the influences of fluoride on the miRNAs and regulated genes in gills. mRNAs were isolated from the gills and the expression profiles were analyzed by using Illumina Hiseq 2500 platforms. Expressions of 7 differentially miRNAs and some related-genes in gills were validated by qRT-PCR. The results showed that miRNAs expressions were notably altered by fluoride. A total of 584 and 327 miRNAs were remarkably changed after 20 and 40 mg/L fluoride exposure, of which 322 were increased and 262 were decreased in 20 mg/L fluoride group, whereas 219 were elevated and 108 were reduced in 40 mg/L fluoride group. The differentially expressive miRNAs confirmed by qRT-PCR were consistent with micro-assay data. Cluster of Orthologous Groups of proteins (COG) function classification showed that the target genes of differentially expressive miRNAs are mainly related to signal transduction mechanisms, replication, transcription, inorganic ion transport and metabolism, repair and recombination, and energy formation and transformation. In addition, fluoride disturbed the expressions of target genes involved in the osmoregulation of the gill in the fluoride-exposed zebrafish, such as the increased expressions of OSTF1 and the decreased expressions of Na⁺-K⁺-ATPase, CFTR, and AQP-3, which provides a possibility that miRNAs regulation induced by fluoride has an effects on osmotic regulation, providing new hints to the osmotic regulatory mechanism of the toxicity caused by fluoride in zebrafish, and distinguishes new biomarkers of miRNAs for fluoride toxicity.

已发现氟化物会对鱼g造成有害影响。尽管在各种新陈代谢活动中起着至关重要的作用，但尚不清楚miRNA是否以及如何参与g中氟化物引起的毒性过程。在这项研究中，雄性斑马鱼暴露于0、20、40 mg / L的氟化物60天，以通过确定氟化物对g中miRNA和调控基因的影响来研究潜在的渗透调节机制。从the中分离mRNA，并使用Illumina Hiseq 2500平台分析表达谱。通过qRT-PCR验证了7中7种差异miRNA和一些相关基因的表达。结果表明，氟化物显着改变了miRNA的表达。暴露于20和40 mg / L的氟化物后，总共584和327个miRNA发生了显着变化，20 mg / L氟化物组增加322个，减少262个，而40 mg / L氟化物组增加219个，减少108个。经qRT-PCR证实的差异表达miRNA与微量分析数据一致。直系同源蛋白质簇的功能分类表明，差异表达miRNA的靶基因主要与信号转导机制，复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化有关。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。40 mg / L氟化物组增加219例，减少108例。经qRT-PCR证实的差异表达miRNA与微量分析数据一致。直系同源蛋白质簇的功能分类表明，差异表达miRNA的靶基因主要与信号转导机制，复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化有关。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。40 mg / L氟化物组增加219例，减少108例。经qRT-PCR证实的差异表达miRNA与微量分析数据一致。直系同源蛋白质簇的功能分类表明，差异表达miRNA的靶基因主要与信号转导机制，复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化有关。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。经qRT-PCR证实的差异表达miRNA与微量分析数据一致。直系同源蛋白质簇的功能分类表明，差异表达miRNA的靶基因主要与信号转导机制，复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化有关。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。经qRT-PCR证实的差异表达miRNA与微量分析数据一致。直系同源蛋白质簇的功能分类表明，差异表达miRNA的靶基因主要与信号转导机制，复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化有关。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。复制，转录，无机离子运输和代谢，修复和重组以及能量形成和转化。此外，氟化物干扰了暴露于氟化物的斑马鱼中ill渗透调节过程中靶基因的表达，例如OSTF1的表达增加和Na的表达减少。⁺ -K ⁺ -ATPase，CFTR和AQP-3，这可能导致氟化物诱导的miRNA调节对渗透调节有影响，这为斑马鱼中氟化物引起的毒性的渗透调节机制提供了新的提示，并区分了miRNA的新生物标志物对氟化物的毒性作用。