Aquaculture ( IF 4.5 ) Pub Date : 2021-02-25 , DOI: 10.1016/j.aquaculture.2021.736545 Shangyu Zhai , Ben Yang , Fuqiang Zhang , Qi Li , Shikai Liu
The Pacific oyster (Crassostrea gigas) is one of the most important aquaculture species in the world, while its industry has been hampered by mass summer mortality caused by pathogenic factors including Vibrio bacteria. Selective breeding of oyster strains with high resistance to Vibrio bacteria would be an effective and sustainable approach to prevent massive economic loss. Estimation of genetic parameters for disease resistance is the critical step toward selective breeding. In this study, we constructed 52 full-sib families using the C. gigas with diverse genetic backgrounds, and performed an artificial infection experiment to assess disease resistance among families and estimate genetic parameters for resistance to Vibrio alginolyticus infection. The survival rate of the 52 families ranged from 0% to 56.25%, suggesting high levels of phenotypic variation in resistance to V. alginolyticus infection. Genetic parameters for resistance to V. alginolyticus infection estimated using six different models revealed low to moderate heritability, ranging from 0.133 to 0.257. The Pearson and Spearman correlation coefficients among family estimated breeding values (EBVs) were high (correlation coefficients ≥0.989), indicating that the predictive ability of different models for family EBVs was consistent. The genetic and phenotypic correlation between resistance to V. alginolyticus and growth traits were low, suggesting the feasibility of simultaneous genetic improvement of both growth and resistance traits. This work reported the first estimation of genetic parameters for resistance to V. alginolyticus and provided valuable information toward genetic improvement of resistance to V. alginolyticus using traditional selection or genomic selection breeding approach.
中文翻译:
估计太平洋牡蛎(Crassostrea gigas)对溶藻弧菌感染的遗传参数。
太平洋牡蛎(Crassostrea gigas)是世界上最重要的水产养殖品种之一,而其产业却受到包括弧菌在内的致病因素导致的夏季大量死亡的困扰。对弧菌具有高度抗性的牡蛎品系的选择性育种将是防止大量经济损失的有效且可持续的方法。估计抗病性的遗传参数是选择性育种的关键步骤。在这项研究中,我们使用具有不同遗传背景的C. gigas构建了52个全同胞家庭,并进行了人工感染实验,以评估各家庭之间的疾病抗性并估计抗性的遗传参数溶藻弧菌感染。52个家庭的成活率在0%至56.25%之间,表明对溶藻弧菌感染的抵抗力表现出高水平的表型变异。使用六个不同模型估算的抗溶藻弧菌感染的遗传参数显示出低至中等的遗传力,范围从0.133至0.257。家庭估计育种值(EBV)之间的Pearson和Spearman相关系数较高(相关系数≥0.989),表明不同模型对家庭EBV的预测能力是一致的。抗溶藻弧菌的遗传和表型相关性且生长性状低,表明同时遗传改良生长性和抗性性状的可行性。这项工作报告了对溶藻弧菌抗性的遗传参数的首次估计,并为使用传统选择或基因组选择育种方法对溶藻弧菌的抗性进行遗传改良提供了有价值的信息。