Laminins (Ln), a type of extracellular matrix glycoprotein, are key regulators of cellular behavior. Recent work revealed that in various tumor cell lines, laminin isoforms influence specific responses, such as cell anchorage, survival, proliferation, migration, organization, and specialization. The contribution of laminin isoforms to the function of gastric cancer cells, however, remain unclear. Here, we revealed that in gastric cancer, laminin isoforms Ln411, Ln421, Ln511, and Ln521 promote cellular proliferation; Ln511 and Ln521 increase cell cytoplasmic volume; Ln511 hampers invadopodia formation in some cells, Ln511 enables prompt adhesion of cells to plates, and Ln411 and Ln511 do not alter the gastric cancer stem cell markers CD44 and Lgr5. These results indicate that Ln411 and Ln511 dynamically modulate the proliferation, adhesion, and morphology of gastric cancer cells in different ways that are independent of stem cell properties. In particular, Ln511 showed a high affinity for gastric cancer cells. Our observations broaden the possible options for controlling cancer cell progression and metastasis by modulating laminin-integrin interactions.

层粘连蛋白 (Ln) 是一种细胞外基质糖蛋白，是细胞行为的关键调节剂。最近的工作表明，在各种肿瘤细胞系中，层粘连蛋白同种型影响特定反应，如细胞锚定、存活、增殖、迁移、组织和特化。然而，层粘连蛋白同种型对胃癌细胞功能的贡献仍不清楚。在这里，我们发现在胃癌中，层粘连蛋白同工型 Ln411、Ln421、Ln511 和 Ln521 促进细胞增殖；Ln511 和 Ln521 增加细胞质体积；Ln511 阻碍某些细胞中的侵袭伪足形成，Ln511 使细胞能够迅速粘附到平板上，并且 Ln411 和 Ln511 不会改变胃癌干细胞标记物 CD44 和 Lgr5。这些结果表明 Ln411 和 Ln511 动态调节增殖、粘附、胃癌细胞以不同方式的形态和形态，这些方式独立于干细胞特性。特别是，Ln511 对胃癌细胞显示出高亲和力。我们的观察通过调节层粘连蛋白-整合素相互作用拓宽了控制癌细胞进展和转移的可能选择。