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Design of a universal primer pair for the identification of deer species
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-10-07 , DOI: 10.1007/s12686-020-01176-2
Liuwei Xie , Yongyan Deng , Xiqun Shao , Pengfei Hu , Dawei Zhao , Chunyi Li , Hengxing Ba

Deer species has both scientific research and economic value, and half of these species, however, are listed as endangered animals. For the conservation purpose, we designed a novel universal deer-specific PCR primer pair based on an evolutionarily conservative coding sequence (i.e., CEP295NL gene) across some deer species. This primer pair was successfully amplified and sequenced, showing around ~ 540 bp in cervids. Validation results showed that it can be utilized to develop a reliable and simple diagnostic tool for distinguishing other closely related species, as well as possibly interspecific identification amongst cervids.



中文翻译:

用于鉴定鹿种的通用引物对的设计

鹿种具有科学研究和经济价值,但是其中一半被列为濒危动物。为了保护起见,我们基于一些鹿物种的进化保守编码序列(即CEP295NL基因)设计了一种新颖的通用鹿特异性PCR引物对。该引物对已成功扩增和测序,在宫颈中显示约540 bp。验证结果表明,它可用于开发一种可靠,简单的诊断工具,以区分其他密切相关的物种,以及可能在种之间进行种间鉴定。

更新日期:2020-10-07
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