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Discovery of SNP markers of red shrimp Aristeus antennatus for population structure in Western Mediterranean Sea
Conservation Genetics Resources ( IF 1.1 ) Pub Date : 2020-11-03 , DOI: 10.1007/s12686-020-01178-0
Gaetano Catanese , Jean Remi Trotta , Mikel Iriondo , Antoni Maria Grau , Andone Estonba

Aristeus antennatus is one of the most exploited and economically important resource for fisheries in the Western and Central Mediterranean Sea displaying low population differentiation with mitochondrial and microsatellite markers. The recent development of Genotyping-by-Sequencing (GBS) methods may contribute to the discovery of SNPs and the assessment of genetic differences between populations of this species for fisheries management. Using samples from four geographical sites in Western Mediterranean and Eastern Atlantic, 115,071 putative SNPs were detected. After the stringent quality control measures and the filtering procedure, 232 SNP loci were discovered. Finally, we selected 80 SNP subset panel for Fluidigm Dynamic array application. The results showed significant differentiation among populations from the four sampling sites. Population assignment power and patterns of population differentiation were comparable between the two SNP panels. These markers represent a useful tool for future genetic application of A. antennatus populations.



中文翻译:

地中海西部红虾触角虾种群结构SNP标记的发现

触角触角是西部和中地中海渔业中最被开发和经济上重要的资源之一,显示出具有线粒体和微卫星标记的低种群分化。通过测序进行基因分型(GBS)方法的最新发展可能有助于发现SNP并评估该物种种群之间的遗传差异以进行渔业管理。使用来自西地中海和东大西洋的四个地理位置的样本,检测到115,071个推定的SNP。经过严格的质量控制措施和过滤程序后,发现了232个SNP位点。最后,我们为Fluidigm动态阵列应用选择了80个SNP子面板。结果显示,这四个采样点的人群之间存在显着差异。两个SNP小组之间的人口分配能力和人口分化模式可比。这些标记代表了未来遗传应用的有用工具。A.触角类种群。

更新日期:2020-11-03
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