As shown in our previous study, Kwoniella heveanensis DMKU-CE82, a volatile organic compound (VOC)-producing yeast, demonstrated promising antagonistic activity against aflatoxin-producing strain of Aspergillus flavus. This yeast’s volatile organic compounds (VOCs) could reduce Aflatoxin B1 (AFB1) in corn grains. In the current study, we evaluated the effect of temperatures and relative humidity on AFB1 reduction during grain storage when co-incubated with this VOC-producing yeast. The VOCs produced by K. heveanensis DMKU-CE82 could promote reduction of AFB1 to less than 20 part per billion (ppb) in the fungal contaminated corn grains under most storage conditions at 35 °C. The major VOCs produced by 2-, 4-, and 6-day-old yeast cultures were closely matched to 3-methyl-1-butanol, 2-methyl-1-butanol, hydrazine-1-1-dimethyl, and butanoic acid-3-methyl. In addition, this yeast strain had the ability to produce β-1,3-glucanase, amylase, cellulase, chitinase, siderophores, and biofilms. Scanning electron microscopy also confirmed the antagonistic activity of K. heveanensis DMKU-CE82 as it caused structural damage to conidia and inhibited the development of mycelia and conidiophores in both direct fungal–yeast interaction and the VOC method in corn grains. These results demonstrated that this yeast strain could be a promising biocontrol agent against aflatoxin-producing fungi in agricultural products.

如在我们以前的研究显示，Kwoniella heveanensis DMKU-CE82，挥发性有机化合物（VOC）产生酵母，证明有希望的拮抗活性的抗黄曲霉毒素产生株黄曲霉。这种酵母的挥发性有机化合物（VOC）可以减少玉米籽粒中的黄曲霉毒素B1（AFB1）。在当前的研究中，我们与该VOC产生酵母共孵育时，评估了温度和相对湿度对谷物储存过程中AFB1减少的影响。heveanensis产生的VOC在大多数温度为35°C的储存条件下，DMKU-CE82可以将真菌污染的玉米谷物中的AFB1降低至十亿分之20（ppb）以下。由2天，4天和6天的酵母培养物产生的主要VOC与3-甲基-1-丁醇，2-甲基-1-丁醇，肼-1--1-二甲基和丁酸紧密匹配-3-甲基。另外，该酵母菌株具有产生β-1，3-葡聚糖酶，淀粉酶，纤维素酶，几丁质酶，铁载体和生物膜的能力。扫描电子显微镜也证实了橡胶树的拮抗活性DMKU-CE82在玉米和谷物的直接真菌-酵母相互作用和VOC方法中都对分生孢子造成了结构破坏，并抑制了菌丝体和分生孢子的发展。这些结果表明，该酵母菌株可能是针对农产品中产生黄曲霉毒素的真菌的有前途的生物防治剂。