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Identification and molecular characterization of rose leaf curl virus in ornamental pomegranate ( Punica granatum L.)
Australasian Plant Pathology ( IF 1.4 ) Pub Date : 2021-02-25 , DOI: 10.1007/s13313-021-00780-6
Kamran Rashid , Mohsin Tariq , Rob W. Briddon , Ioly Kotta-Loizou , Khadim Hussain

Symptoms of leaf curling and yellowing, typical of begomoviruses, were observed on ornamental pomegranate plants in a park in Faisalabad, Pakistan. Leaves from symptomatic plants were collected and DNA extracted. PCR amplification with universal primers for the amplification of begomoviruses amplified an approx. 2.8 kb fragment which was cloned and sequenced. Similar amplifications with primers for begomovirus DNA B component and betasatellites were uniformly negative and no amplifications were obtained with DNA extracted from a non-symptomatic pomegranate plant. Sequence comparisons showed the cloned fragment to be an isolate of rose leaf curl virus (RoLCuV). This is the first time RoLCuV has been identified in a bushy shrub pomegranate. Sequence analysis shows the RoLCuV isolate from pomegranate to have a recombination history distinct from other RoLCV isolates.



中文翻译:

观赏石榴(Punica granatum L.)中玫瑰叶片卷曲病毒的鉴定和分子特征

在巴基斯坦费萨拉巴德的一个公园里,在观赏性石榴植物上观察到了典型的begomoviruses的卷曲和发黄症状。收集有症状植物的叶子并提取DNA。用通用引物进行PCR扩增,可扩增约2000株begomoviruses。克隆并测序了2.8 kb的片段。用begomovirus DNA B组分和β卫星引物进行的相似扩增均呈阴性,从无症状石榴植物中提取的DNA均未获得扩增。序列比较显示克隆的片段是玫瑰叶片卷曲病毒(RoLCuV)的分离物。这是首次在浓密灌木石榴中鉴定出RoLCuV。

更新日期:2021-02-25
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