The long-chain noncoding RNA ADAMTS9-AS2 functions as a tumor suppressor gene in many cancers. However, the underlying mechanism remains to be fully elucidated in bladder cancer (BC). ADAMTS9-AS2 exhibited a lower expression level in BC samples and cell lines. In addition, overexpression of ADAMTS9-AS2 obviously suppressed proliferation and migration, and induced apoptosis of T24 cells, while transfection with the ADAMTS9-AS2 inhibitor had opposite results in 5637 cells. Furthermore, miR-182-5p was the target microRNA of ADAMTS9-AS2 and was negatively correlated with ADAMTS9-AS2 expression. Upregulation of miR-182-5p reversed the effects of ADAMTS9-AS2 overexpression on biological function in T24 cells. ADAMTS9-AS2 was a tumor suppressor that inhibited BC cell proliferation and induced cellular apoptosis by targeting miR-182-5p, and it could be a promising target for BC treatment.

中文翻译：

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长链非编码 RNA ADAMTS9-AS2 通过调控 miR-182-5p 调控膀胱癌细胞的增殖、迁移和凋亡

长链非编码 RNA ADAMTS9-AS2 在许多癌症中充当肿瘤抑制基因。然而，膀胱癌（BC）的潜在机制仍有待完全阐明。ADAMTS9-AS2 在 BC 样品和细胞系中表现出较低的表达水平。此外，ADAMTS9-AS2的过表达明显抑制了T24细胞的增殖和迁移，并诱导了T24细胞的凋亡，而ADAMTS9-AS2抑制剂的转染在5637细胞中具有相反的结果。此外，miR-182-5p 是 ADAMTS9-AS2 的靶标 microRNA，与 ADAMTS9-AS2 表达呈负相关。miR-182-5p 的上调逆转了 ADAMTS9-AS2 过表达对 T24 细胞生物学功能的影响。ADAMTS9-AS2 是一种肿瘤抑制因子，通过靶向 miR-182-5p 抑制 BC 细胞增殖并诱导细胞凋亡，