Spruce budworm, Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae), is a destructive defoliator found throughout the Nearctic boreal forest. This pest has a broad geographic range and shows regional variation in key life history traits. These population differences may represent important adaptations to local environmental conditions and reflect underlying genetic diversity. Existing laboratory colonies of spruce budworm do not capture this regional variation, so we established five new spruce budworm colonies from across its range to explore regional adaptations among spruce budworm populations within common garden experiments. We present methods for establishing new spruce budworm laboratory colonies from wild populations. We describe the process of flushing, rearing, and disease screening used on these new populations to produce healthy disease-free laboratory stocks.

云杉芽虫，Choristoneura fumiferana（Clemens）（鳞翅目：Tortricidae），是一种破坏性的落叶者，遍布整个北北极森林。这种有害生物地理范围广，在主要的生活史特征上显示出区域差异。这些人口差异可能代表着对当地环境条件的重要适应，并反映了潜在的遗传多样性。现有的云杉芽虫实验室菌落无法捕获这种区域变异，因此我们在其整个范围内建立了五个新的云杉芽虫菌落，以探索普通花园实验中云杉芽虫种群之间的区域适应性。我们提出了从野生种群中建立新的云杉芽虫实验室菌落的方法。我们描述了在这些新种群上使用的冲洗，饲养和疾病筛查的过程，以生产健康，无病的实验室种群。