Modern patterning techniques enable the creation of highly controlled protein microenvironments, providing analysis tools for cell biology and scaffolds for tissue engineering. Thomas Scheibel and colleagues review surface nano‐ and microstructuring of protein‐based materials and the patterning techniques diversely applied on protein‐based scaffolds. Strategies include patterning of proteins, for example, through the controlled formation of β‐sheets and the use of photosensitive residues. Globular proteins (e.g., enzymes, growth factors and fluorescent proteins) can be patterned using charges on the protein's surface or other chemical entities, and by introducing bio‐orthogonal site‐specific modifications such as polymer brushes or oligonucleotides to achieve spatiotemporal immobilization. (DOI: 10.1002/bip.23412)

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现代图案技术可创建高度受控的蛋白质微环境，从而为细胞生物学和组织工程支架提供分析工具。Thomas Scheibel及其同事回顾了蛋白质基材料的表面纳米结构和微观结构，以及在蛋白质基支架上广泛应用的构图技术。策略包括通过控制β-折叠的形成和使用光敏残基来形成蛋白质图案。可以使用蛋白质表面或其他化学实体上的电荷，并通过引入生物正交位点特异性修饰（例如聚合物刷或寡核苷酸）来实现球形蛋白（例如酶，生长因子和荧光蛋白）的模式化，以实现时空固定。（DOI：10.1002 / bip.23412）