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Chromosome analysis of walking catfish, Clarias magur (Teleostei, Siluriformes, Clariidae), using staining, FISH with 18S, 5S and BAC probes
Journal of Applied Ichthyology ( IF 0.9 ) Pub Date : 2021-02-24 , DOI: 10.1111/jai.14188
Vishwamitra Baisvar 1 , Basdeo Kushwaha 1 , Ravindra Kumar 1 , Murali Sanjeev Kumar 1 , Manmohan Pandey 1 , Mahender Singh 1
Affiliation  

The chromosomal characteristics of Clarias magur were examined using conventional (Giemsa-staining, Ag-impregnation and CMA3 + DAPI fluorescence) and molecular/ FISH (18S & 5S rDNA probes + one BAC DNA probe) cytogenetic tools. The diploid chromosome number was 50 and the karyotype consisted of 14 metacentric, 20 sub-metacentric, 8 sub-telocentric, 8 acrocentric chromosomes with 84 chromosome arms without any heteromorphic pair. The C-heterochromatic blocks were located on centromeric position of 13 pairs of chromosomes. The NOR sites, visualized by AgNO3- and CMA3- staining, were situated at p arms of chromosome pair No. 21, which also corresponded to 18S rDNA site visualized by FISH. The FISH signal of ICF_001_D19 clone probe was observed on 18th chromosome pair. The findings of the present study on C. magur provided valuable markers for the chromosome identification and locations of genes of the BAC clone on the chromosome will lead to the construction of physical map of genome of this species.

中文翻译:

使用染色法、使用 18S、5S 和 BAC 探针的 FISH 对步行鲶鱼(鲶科、鲶科、鲶科)进行染色体分析

使用常规(吉姆萨染色、银浸渍和 CMA 3  + DAPI 荧光)和分子/ FISH(18S 和 5S rDNA 探针 + 一种 BAC DNA 探针)细胞遗传学工具检查Clarias magur的染色体特征。二倍体染色体数为50,核型由14条中着丝粒、20条亚中着丝粒、8条亚端着丝粒、8条近着丝粒染色体和84条染色体臂组成,没有任何异形对。C-异色块位于13对染色体的着丝粒位置。NOR 位点,由 AgNO 3 - 和 CMA 3可视化- 染色,位于第 21 号染色体对的 p 臂,这也对应于 FISH 可视化的 18S rDNA 位点。在第18对染色体上观察到ICF_001_D19克隆探针的FISH信号。在本研究的结果magur C.对染色体鉴定和染色体上的BAC克隆的基因的位置提供了宝贵的标记将导致这一物种的基因组物理图谱的构建。
更新日期:2021-02-24
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