当前位置: X-MOL 学术Environ. Int. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Size-dependent adverse effects of microplastics on intestinal microbiota and metabolic homeostasis in the marine medaka (Oryzias melastigma)
Environment International ( IF 11.8 ) Pub Date : 2021-02-24 , DOI: 10.1016/j.envint.2021.106452
Xu Zhang , Kai Wen , Dongxiao Ding , Jintao Liu , Zhao Lei , Xiaoxuan Chen , Guozhu Ye , Jie Zhang , Heqing Shen , Changzhou Yan , Sijun Dong , Qiansheng Huang , Yi Lin

Microplastic (MP) is an emerging environmental pollutant and exposure to MPs has been associated with numerous adverse health outcomes in both wild and laboratory animals. The toxicity of MPs depends on concentration, exposure time, chemical composition and size distribution, but the impacts of particle size remain inconclusive yet. In this study, adult marine medaka (Oryzias melastigma) were exposed to different size of polystyrene MPs (PS-MPs) with concentration of 10 mg/L for 60 days and the growth performance, lipid metabolism, immune parameters and gut microbiome were determined. Results indicated that particle size is a dominant factor causing lipid metabolism disorders and hepatic toxicity in PS-MPs-exposed fish. The bodyweight, adipocyte size and hepatic lipid contents were significantly increased in 200 μm PS-MPs-exposed fish, while 2 and 10 μm PS-MPs-exposed fish exhibited liver injury principally manifested as the presence of little fibrosis and inflammation. Given that larger particles could not enter the circulatory system, the impacts of PS-MPs on intestinal microbial biota homeostasis were further investigated. The results not only showed the characterization of gut microbial communities in Oryzias melastigma, but also indicated that microbial diversity and composition were altered in gut of fish exposed to PS-MPs, in particular 200 μm PS-MPs. The differentially abundant bacterial taxa in PS-MPs-exposed fish mainly belonged to the phylum Verrucomicrobia, Firmicutes and Fusobacteria. And furthermore, increased abundance of Verrucomicrobia and Firmicutes/Bacteroidetes ratio and decreased Fusobacteria were correlated with the increased bodyweight. Intestinal microbiome should play a critical role in regulating host lipid metabolism in fish exposed to lager size of PS-MPs.



中文翻译:

微型塑料对海洋中高加索Oryzias melastigma)肠道菌群和代谢体内稳态的大小依赖性不利影响

微塑料(MP)是一种新兴的环境污染物,在野生和实验动物中,接触MP都会导致许多不良健康后果。MPs的毒性取决于浓度,暴露时间,化学成分和粒径分布,但粒径的影响尚无定论。在这项研究中,成年海洋(Oryzias melastigma)暴露于浓度为10 mg / L的不同尺寸的聚苯乙烯MPs(PS-MPs)中60天,并测定其生长性能,脂质代谢,免疫参数和肠道微生物组。结果表明,粒径是引起PS-MPs暴露的鱼类脂质代谢紊乱和肝毒性的主要因素。的体重,脂肪细胞尺寸和肝脏脂质含量在200微米PS-MPS-暴露鱼显著增加,而在2和10微米的PS-MPS-暴露鱼表现出的肝损伤主要表现为 所述 的存在  纤维化和炎症。考虑到较大的颗粒无法进入循环系统,进一步研究了PS-MP对肠道菌群生物稳态的影响。结果不仅显示了稻曲肠肠道微生物群落的特征,而且还表明暴露于PS-MPs,特别是200μmPS -MPs的鱼类的肠道中微生物的多样性和组成发生了变化。暴露于PS-MPs的鱼类中细菌丰富的分类群主要属于Verrucomicrobi a门,FirmicutesFusobacteria。而且,VerrucomicrobiaFirmicutes / Bacteroidetes的丰度增加了比率和梭菌减少与体重增加有关。肠道微生物组在调节暴露于较大PS-MPs的鱼类中的宿主脂质代谢中起着至关重要的作用。

更新日期:2021-02-24
down
wechat
bug