Repetitive DNAs comprise large portion of eukaryote genomes. In genome projects, the assembly of repetitive DNAs is challenging due to the similarity between repeats, which generate ambiguities for alignment. Fluorescence in situ hybridization (FISH) is a powerful technique for the physical mapping of various sequences on chromosomes. This technique is thus very helpful in chromosome-based genome assemblies, providing information on the fine architecture of genomes and their evolution. However, various protocols are currently used for FISH mapping, most of which are relatively laborious and expensive, or work properly only with a specific type of probes or sequences, and there is a need for a universal and affordable FISH protocol. Here we tested a FISH protocol for mapping of different DNA repeats, such as multigene families (rDNAs, U snDNAs, histone genes), satellite DNAs, microsatellites, transposable elements, DOP-PCR products, and telomeric motif (TTAGG)_n, on the chromosomes of various insects and other arthropods. Different cell types and stages obtained from diverse tissues were used. The FISH procedure proved high quality and reliable results in all experiments performed. We obtained data on the chromosomal distribution of DNA repeats in representatives of insects and other arthropods. Thus, our results allow us to conclude that the protocol is universal and requires only time adjustment for chromosome/DNA denaturation. The use of this FISH protocol will facilitate studies focused on understanding the evolution and role of repetitive DNA in arthropod genomes.

重复DNA包含大部分的真核生物基因组。在基因组计划中，由于重复序列之间的相似性，重复DNA的组装具有挑战性，而重复序列之间的相似性会产生歧义。荧光原位杂交（FISH）是对染色体上各种序列进行物理定位的有力技术。因此，该技术在基于染色体的基因组组装中非常有用，可提供有关基因组的精细结构及其进化的信息。但是，目前有多种协议用于FISH定位，其中大多数比较费力且昂贵，或者仅适用于特定类型的探针或序列才能正常工作，因此需要一种通用且负担得起的FISH协议。在这里，我们测试了一种FISH协议，用于定位不同的DNA重复序列，例如多基因家族（rDNA，U snDNA，_n，位于各种昆虫和其他节肢动物的染色体上。使用了从不同组织获得的不同细胞类型和阶段。在所有进行的实验中，FISH程序均证明了高质量和可靠的结果。我们获得了有关昆虫和其他节肢动物代表中DNA重复序列的染色体分布的数据。因此，我们的结果使我们可以得出结论，该协议是通用的，并且只需要调整染色体/ DNA变性的时间即可。这种FISH方案的使用将有助于专注于理解节肢动物基因组中重复DNA的进化和作用的研究。