A method for regeneration via direct organogenesis in Lilium cv. Pavia using leaf primary explants has been established. Outer leaves from in vitro plantlets of 10 to 12 cm were used as the primary explant for direct organogenesis. Shoots were directly regenerated from leaf explants within 25 d of culture on Murashige and Skoog (MS) augmented with 4.5 μM 2, 4-dichlorophenoxyacetic acid (2, 4-D), 5.4 μM α-naphthaleneacetic acid (NAA), and 2.2 μM 6-benzylaminopurine (BAP). Different regions of the same leaf explant exhibited varied responses. The acropetal 3 to 4 cm of the leaf (L1) produced the maximum number of shoots (75%) indicating its high regeneration ability whereas the response of middle portion (L2) and basipetal portion of the leaf (L3) exhibited a significantly decreasing trend of shoot induction with 37.5 and 12.5%, respectively. HPLC analysis of endogenous indole-3-acetic acid (IAA) concentration established significantly higher concentration in the acropetal segments (L1). The in vitro regenerated plants were rooted in half strength MS media and were successfully hardened and field transferred. Our study established that different regions of the same leaf produce significantly different responses for shoot induction and this is attributed to the variable levels of endogenous IAA. This quick method of direct organogenesis can be applied for commercial multiplication of this important hybrid.

一种通过直接在百合属植物中发生器官再生的方法。已经建立了使用叶初级外植体的帕维亚。体外叶片10至12厘米的小植株用作直接器官发生的主要外植体。在培养了25 d的Murashige和Skoog（MS）上，直接从叶片外植体中再生芽，并加入4.5μM2、4-二氯苯氧基乙酸（2，4-D），5.4μMα-萘乙酸（NAA）和2.2μM 6-苄氨基嘌呤（BAP）。同一叶片外植体的不同区域表现出不同的响应。顶叶的3至4 cm叶片（L1）产生了最多的芽（75％），表明其具有较高的再生能力，而叶片的中间部分（L2）和基底基部（L3）的响应却呈现出明显下降的趋势芽诱导率分别为37.5和12.5％。内源性吲哚-3-乙酸（IAA）浓度的HPLC分析建立了较高的顶段（L1）浓度。这体外再生植物植根于半强度MS培养基中，并成功硬化并转移到田间。我们的研究确定同一叶的不同区域对芽诱导产生明显不同的响应，这归因于内源IAA的可变水平。这种直接器官发生的快速方法可以用于这种重要杂种的商业繁殖。