Abstract

Studies were conducted to obtain a complex enzyme preparation that allows efficient hydrolysis of the main plant nonstarch polysaccharides (cellulose, xylans, and pectin). The goal of the studies was to optimize the composition of a fermentation medium for the submerged cultivation of the new mutant strain T. reesei Co-44, a highly active producer of endo-carbohydrases. A concentrate of low molecular soy components was chosen as the inducer of the key carbohydrase biosynthesis by the strain. This concentrate provided the maximum (more than eightfold) increase in polygalacturonase activity and an increased level of endoglucanase and xylanase biosynthesis. After the cultivation of T. reesei Co-44 under optimized conditions, a complex enzyme preparation, Xylorizin K4, was obtained, and its physicochemical properties were studied. The presence of endopolygalacturonase (GH28) T. reesei in Xylorizin K4 was confirmed via electrophoresis and MALDI⎯TOF mass spectrometry. The studies show the potential of Xylorizin K4 application for the production of soy-protein concentrates to eliminate the main soybean nonstarch polysaccharides.

中文翻译：

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基于新型突变菌株里氏木霉Co-44的具有增强的果胶酶活性的复合酶制剂的获得

摘要

进行了研究以获得复杂的酶制剂，该酶制剂可有效水解主要植物的非淀粉多糖（纤维素，木聚糖和果胶）。研究的目的是优化用于新突变菌株里氏木霉Co-44（一种高活性内糖化酶生产商）的水下培养的发酵培养基的组成。选择低分子大豆成分的浓缩物作为该菌株关键糖酶生物合成的诱导物。该浓缩物提供了聚半乳糖醛酸酶活性的最大增加（超过八倍），以及内切葡聚糖酶和木聚糖酶生物合成的水平增加。里氏木霉栽培后在优化条件下制备了Co-44，制备了复合酶制剂木糖苷K4，并对其理化性质进行了研究。通过电泳和MALDI⎯TOF质谱法证实了木糖内酯K4中存在内聚半乳糖醛酸酶（GH28）里氏木霉。研究表明，使用Xylorizin K4生产大豆浓缩蛋白可以消除主要的大豆非淀粉多糖。