ABSTRACT

In the present study, we examined the effect of cold-water swimming stress (CWSS) on plasma corticosterone levels. Mice were exposed to stress in 4°C for 3 mins. Plasma corticosterone (CORT) level was measured at 0, 15, and 30 min after stress stimulation. The plasma CORT level was gradually increased up to 30 min. Then we further examined the changes of several signaling molecules expression levels, such as p-ERK, p-JNK, p-P38, p-AMPKα1, p-AMPKα2, and p-mTOR, in the HPA axis. We observed that those signaling molecules were altered after stress in the HPA axis. p-ERK, p-JNK, p-P38, and p-mTOR proteins expression were reduced by CWSS in the HPA axis. However, the phosphorylation of AMPKα1 and AMPKα2 were activated after CWSS in the HPA axis. Our results suggest that the upregulation of plasma CORT level induced by CWSS may be modulated by the those signaling molecules.

摘要

在本研究中，我们检查了冷水游泳压力（CWSS）对血浆皮质酮水平的影响。将小鼠在4℃下暴露3分钟。在应激刺激后第0、15和30分钟测量血浆皮质激素（CORT）水平。血浆CORT水平逐渐升高至30分钟。然后，我们进一步检查了HPA轴上几种信号分子表达水平的变化，例如p-ERK，p-JNK，p-P38，p-AMPKα1，p-AMPKα2和p-mTOR。我们观察到那些信号分子在HPA轴受力后发生了变化。在HPA轴上，CWSS降低了p-ERK，p-JNK，p-P38和p-mTOR蛋白的表达。然而，在HPA轴上进行CWSS后，AMPKα1和AMPKα2的磷酸化被激活。