当前位置: X-MOL 学术Biofouling › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The novel endolysin XZ.700 effectively treats MRSA biofilms in two biofilm models without showing toxicity on human bone cells in vitro
Biofouling ( IF 2.7 ) Pub Date : 2021-02-21 , DOI: 10.1080/08927014.2021.1887151
Jesse W. P Kuiper 1 , Jolanda M. A Hogervorst 2 , Bjorn L. Herpers 3 , Astrid D. Bakker 2 , Jenneke Klein-Nulend 2 , Peter A. Nolte 1 , Bastiaan P. Krom 4
Affiliation  

Abstract

In this in vitro study the effect of XZ.700, a new endolysin, on methicillin resistant Staphylococcus aureus (MRSA) biofilms grown on titanium was evaluated. Biofilms of S. aureus USA300 were grown statically and under flow, and treatment with XZ.700 was compared with povidone-iodine (PVP-I) and gentamicin. To evaluate the cytotoxic effects of XZ.700 and derived biofilm lysates, human osteocyte-like cells were exposed to biofilm supernatants, and metabolism and proliferation were quantified. XZ.700 showed a significant, concentration dependent reduction in biofilm viability, compared with carrier controls. Metabolism and proliferation of human osteocyte-like cells were not affected by XZ.700 or lysates, unlike PVP-I and gentamicin lysates which significantly inhibited proliferation. Using time-lapse microscopy, rapid biofilm killing and removal was observed for XZ.700. In comparison, PVP-I and gentamicin showed slower biofilm killing, with no apparent biofilm removal. In conclusion, XZ.700 reduced MRSA biofilms, especially under flow condition, without toxicity for surrounding bone cells.



中文翻译:

新型内溶素XZ.700可有效治疗两种生物膜模型中的MRSA生物膜,而不会在体外对人骨细胞产生毒性

摘要

在这项体外研究中,评估了新的内溶素XZ.700对在钛上生长的耐甲氧西林的金黄色葡萄球菌(MRSA)生物膜的影响。金黄色葡萄球菌的生物膜USA300静态生长并流动,然后将XZ.700的处理与聚维酮碘(PVP-1)和庆大霉素进行比较。为了评估XZ.700和衍生的生物膜裂解物的细胞毒性作用,将人骨细胞样细胞暴露于生物膜上清液,并对新陈代谢和增殖进行定​​量。与载体对照相比,XZ.700显示出生物膜活力的显着浓度依赖性降低。人骨细胞样细胞的代谢和增殖不受XZ.700或裂解物的影响,不像PVP-1和庆大霉素裂解物显着抑制增殖。使用延时显微镜,对XZ.700观察到快速的生物膜杀死和去除。相比之下,PVP-1和庆大霉素显示出较慢的生物膜杀灭,没有明显的生物膜去除。总之,XZ.700减少了MRSA生物膜,

更新日期:2021-03-27
down
wechat
bug