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Tumor‐Associated Macrophage and Tumor‐Cell Dually Transfecting Polyplexes for Efficient Interleukin‐12 Cancer Gene Therapy
Advanced Materials ( IF 29.4 ) Pub Date : 2021-02-22 , DOI: 10.1002/adma.202100137
Nasha Qiu , Guowei Wang , Jinqiang Wang , Quan Zhou , Mengyu Guo , Yaling Wang , Xuhao Hu , Huige Zhou , Ru Bai , Min You , Zhen Zhang , Chunying Chen , Ying Liu , Youqing Shen

Adv. Mater. 2020, 32, 2001689

In the originally published article, the wrong image for the collagen staining of the Lipo‐2000/pIL12‐treated tumor was used. A duplicate of the image for the PEI/PIL12 group was selected in error.

The revised figure is shown below and the corrected subpanel, row 3, column 6, is marked by the black box.

image
Figure 4
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Treatment‐induced modulation of the tumor microenvironment. The KPC tumors were inoculated and treated four times, as Figure 3E, and then dissected on 12 days after the last treatment; each tumor was digested to isolate all the cells for flow cytometry analysis and was sectioned and stained for immunofluorescence imaging. A) The relative immune cell populations out of the tumor's total cells analyzed by flow cytometry (n = 6). B) The cytokines’ mRNA levels relative to the PBS control in the tumors analyzed by quantitative RT‐PCR (n = 6). C) Immunohistochemical staining and Masson's Trichrome staining of the KPC tumors for analyzing the antitumor markers and tumor progression markers. Tumor sections were 5 µm thick. The black dotted lines outline the large acellular cracks and fragmentation in the tumor. Scale bars are 100 µm in immunofluorescence imaging and 200 µm in the Masson's Trichrome staining imaging. (***P < 0.001; **P < 0.01; *P < 0.05; NS, not significant.).


中文翻译:

肿瘤相关巨噬细胞和肿瘤细胞双重转染复合物,用于高效白介素-12癌症基因治疗。

进阶 母校 202032,2001689

在最初发表的文章中,使用了Lipo-2000 / pIL12处理的肿瘤的胶原蛋白染色错误的图像。错误选择了PEI / PIL12组的图像副本。

修改后的图如下所示,校正后的子面板第3行第6列用黑框标记。

图像
图4
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治疗诱导的肿瘤微环境调节。如图3E所示,将KPC肿瘤接种并治疗四次,然后在最后一次治疗后12天进行解剖;消化每种肿瘤以分离所有细胞用于流式细胞术分析,并切片和染色以进行免疫荧光成像。A)通过流式细胞仪分析的肿瘤总细胞中的相对免疫细胞群(n  = 6)。B)细胞因子的mRNA水平相对于在肿瘤中与PBS对照分析通过定量RT-PCR(Ñ = 6)。C)KPC肿瘤的免疫组织化学染色和Masson三色染色,用于分析抗肿瘤标志物和肿瘤进展标志物。肿瘤切片为5μm厚。黑色虚线概述了肿瘤中较大的无细胞裂纹和碎片。比例尺在免疫荧光成像中为100 µm,在Masson Trichrome染色成像中为200 µm。(*** P  <0.001; ** P  <0.01; * P  <0.05; NS,不显着。)。
更新日期:2021-02-22
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