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Environmental concentrations of antibiotics alter the zebrafish gut microbiome structure and potential functions
Environmental Pollution ( IF 6.792 ) Pub Date : 2021-02-22 , DOI: 10.1016/j.envpol.2021.116760
Masood ur Rehman Kayani; Kan Yu; Yushu Qiu; Yao Shen; Caixia Gao; Ru Feng; Xinxin Zeng; Weiye Wang; Lei Chen; Huang Li Su

A paradoxical impact of high rates of production and consumption of antibiotics is their widespread release in the environment. Consequently, low concentrations of antibiotics and their byproducts have been routinely identified from various environmental settings especially from aquatic environments. However, the impact of such low concentrations of antibiotics on the exposed host especially in early life remains poorly understood. We exposed zebrafish to two different environmental concentrations of oxytetracycline and sulfamethoxazole, from larval stage to adulthood (∼120 days) and characterized their impact on the taxonomic diversity, antibiotic resistance genes, and metabolic pathways of the gut microbiome using metagenomic shotgun sequencing and analysis. Long term exposure of environmental concentrations of oxytetracycline and sulfamethoxazole significantly impacted the taxonomic composition and metabolic pathways of zebrafish gut microbiome. The antibiotic exposed samples exhibited significant enrichment of multiple flavobacterial species, including Flavobacterium sp. F52, Flavobacterium johnsoniae and Flavobacterium sp. Fl, which are well known pathogenic bacteria. The relative abundance of antibiotic resistance genes, especially several tetratcycline and sulfonamide resistance genes were significantly higher in the exposed samples and showed a linear correlation with the antibiotic concentrations. Furthermore, several metabolic pathways, including folate biosynthesis, oxidative phosphorylation, and biotin metabolism pathways, showed significant enrichment in the antibiotic exposed samples. Collectively, our results suggest that early life exposure of the environmental concentrations of antibiotics can increase the abundance of unfavorable bacteria, antibiotic resistance genes and associated pathways in the gut microbiome of zebrafish.



中文翻译:

环境浓度的抗生素会改变斑马鱼肠道微生物组的结构和潜在功能

抗生素的高生产率和高消耗率的矛盾之处在于它们在环境中的广泛释放。因此,常规地从各种环境设置中,尤其是从水生环境中识别出低浓度的抗生素及其副产物。然而,如此低浓度的抗生素对暴露的宿主的影响,尤其是在生命早期,仍然知之甚少。从幼虫阶段到成年期(约120天),我们将斑马鱼暴露于两种不同浓度的土霉素和磺胺甲恶唑,并使用宏基因组met弹枪测序和分析来表征斑马鱼对肠道菌群的分类学多样性,抗生素抗性基因和代谢途径的影响。长期暴露于环境浓度的土霉素和磺胺甲基异恶唑显着影响了斑马鱼肠道微生物组的分类学组成和代谢途径。暴露于抗生素的样品表现出多种黄细菌的显着富集,包括黄杆菌属 F52,约翰逊杆菌黄杆菌sp。F1是众所周知的致病细菌。在暴露的样品中,抗生素抗性基因的相对丰度,尤其是几个四环素和磺酰胺抗性基因的相对丰度明显更高,并且与抗生素浓度呈线性关系。此外,一些代谢途径,包括叶酸生物合成,氧化磷酸化和生物素代谢途径,在暴露于抗生素的样品中显示出明显的富集。总体而言,我们的结果表明,早期暴露于环境浓度的抗生素可增加斑马鱼肠道微生物组中不利细菌的数量,抗生素抗性基因和相关途径。

更新日期:2021-02-22
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