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Preferential water uptake and differences in the anatomical structure of the distal end of grape berry may jointly lead to cracking in vitro soaking
Horticulture, Environment, and Biotechnology ( IF 2.4 ) Pub Date : 2021-02-22 , DOI: 10.1007/s13580-020-00324-9
Chuan Zhang , Liwen Cui , Chonghuai Liu , Xiucai Fan , Jinggui Fang

The distal end of grape berries preferentially cracks during the soaking process in vitro, which may be related to preferential water uptake and cell anatomy. Thus, the relationship between water uptake and cell anatomical structure was assessed using 49 grape varieties. In vitro immersion experiments were performed on mature berries to measure the differences in berry-cracking properties. A whole-berry in vitro staining method was used to trace the water uptake of the vascular bundles, and paraffin sections were used to observe and analyze the structural parameters of the different tissues. The results showed that the berry-cracking rate and water uptake of the distal end of berries prone to cracking were significantly higher than that of the resistant berries. Compared with the berries that were resistant to cracking, the berries that were prone to cracking possessed a thin cuticle, epidermis and sub-epidermis. Dye accumulated in the distal end of the cracking-prone berries, which even exhibited cracking during the dyeing process. However, less dye accumulated on the surface of the cracking-resistant varieties and was only sporadically distributed on the surface. In addition, cracking of the distal end of the berry may also be related to other shape and size parameters of the cells in cell layer. Analysis of vascular bundle water transport and the differences in the cell structure characteristics of the different varieties, offered a preliminary explanation for why the distal end of the berry is prone to cracking, providing theoretical support for further research on the mechanisms of berry cracking.



中文翻译:

优先吸水和葡萄浆果远端的解剖结构差异可能共同导致体外浸泡破裂

葡萄浆果的远端在体外浸泡过程中优先裂开,这可能与优先吸水和细胞解剖结构有关。因此,使用49个葡萄品种评估了水分吸收与细胞解剖结构之间的关系。在成熟浆果上进行了体外浸泡实验,以测量浆果裂解特性的差异。采用全浆果体外染色法追踪血管束的吸水量,并用石蜡切片观察和分析不同组织的结构参数。结果表明,易开裂的浆果远端的开裂率和吸水率明显高于抗性浆果。与抗裂的浆果相比,容易破裂的浆果具有较薄的表皮,表皮和亚表皮。染料积聚在易开裂浆果的远端,甚至在染色过程中也表现出开裂。然而,较少的染料积累在抗裂品种的表面上,并且仅散布在表面上。另外,浆果远端的破裂也可能与细胞层中细胞的其他形状和大小参数有关。对维管束水运输和不同品种细胞结构特征的差异的分析,为为什么浆果的远端易于破裂提供了初步解释,为进一步研究浆果破裂的机理提供了理论支持。表皮和亚表皮。染料积聚在易开裂浆果的远端,甚至在染色过程中也表现出开裂。然而,较少的染料积累在抗裂品种的表面上,并且仅散布在表面上。另外,浆果远端的破裂也可能与细胞层中细胞的其他形状和大小参数有关。对维管束水运输和不同品种细胞结构特征的差异的分析,为为什么浆果的远端易于破裂提供了初步解释,为进一步研究浆果破裂的机理提供了理论支持。表皮和亚表皮。染料积聚在易开裂浆果的远端,甚至在染色过程中也表现出开裂。然而,较少的染料积累在抗裂品种的表面上,并且仅散布在表面上。另外,浆果远端的破裂也可能与细胞层中细胞的其他形状和大小参数有关。对维管束水运输和不同品种细胞结构特征的差异的分析,为浆果远端易开裂的原因提供了初步解释,为进一步研究浆果开裂的机理提供了理论支持。然而,较少的染料积累在抗裂品种的表面上,并且仅散布在表面上。另外,浆果远端的破裂也可能与细胞层中细胞的其他形状和大小参数有关。对维管束水运输和不同品种细胞结构特征的差异的分析,为为什么浆果的远端易于破裂提供了初步解释,为进一步研究浆果破裂的机理提供了理论支持。然而,较少的染料积累在抗裂品种的表面上,并且仅散布在表面上。另外,浆果远端的破裂也可能与细胞层中细胞的其他形状和大小参数有关。对维管束水运输和不同品种细胞结构特征的差异的分析,为为什么浆果的远端易于破裂提供了初步解释,为进一步研究浆果破裂的机理提供了理论支持。

更新日期:2021-02-22
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