ABSTRACT

Bend neck of scape and short vase life are the most important problems of cut gerbera flowers in postharvest stage. Inhibitors of laccase enzyme influence lignin formation at cut stem end of flowers. In this study, cut gerbera (Gerbera jamesonii cv. Intense) flowers were subjected to laccase enzyme inhibitors including Dithiothreitol (DTT: 0.05, 0.1, 0.2 mM), Thioglycolic acid (TGA: 0.25, 0.5, 1 mM), or Cetyltrimethylammonium bromide (CTAB: 8, 10, 12 mM) for 20 h as pulse treatment. Results showed that 0.2 mM DTT prevented stem bending appropriately. Also, increased the vase life, maintained relative fresh weight, improved water uptake, inhibited laccase activity, and delayed the anthocyanin degradation and lignin formation. Treatment of 0.5 mM TGA had a desirable effect in extending vase life and control of stem bending. However, CTAB had negative effects on vase life of cut gerbera flowers. Thus, the presence of inhibitors in vase solution had a positive effect on control of lignin formation in end of cut stems. Our results confirmed a decrease in lignin formation at cut stem end by DTT 0.2 mM and TGA 0.5 mM. This study suggested that laccase enzyme inhibitors in vase solution are sufficient to maintain water flow process and control stem bending in cut gerbera flowers.

摘要

花梗弯曲和瓶插寿命短是非洲菊切花采后最重要的问题。漆酶抑制剂影响花茎端木质素的形成。在本研究中，切开非洲菊（Gerbera jamesonii简历。强烈的）花受到漆酶抑制剂的影响，包括二硫苏糖醇（DTT：0.05、0.1、0.2 mM）、硫代乙醇酸（TGA：0.25、0.5、1 mM）或十六烷基三甲基溴化铵（CTAB：8、10、12 mM） h 作为脉冲治疗。结果表明，0.2 mM DTT 适当地防止了茎弯曲。此外，增加了瓶插寿命，保持了相对鲜重，提高了吸水率，抑制了漆酶活性，并延迟了花青素降解和木质素的形成。0.5 mM TGA 的处理在延长花瓶寿命和控制茎弯曲方面具有理想的效果。然而，CTAB 对非洲菊切花的瓶插寿命有负面影响。因此，花瓶溶液中抑制剂的存在对控制切茎末端木质素的形成有积极的影响。我们的结果证实了 DTT 0.2 mM 和 TGA 0.5 mM 在切茎末端木质素形成的减少。该研究表明，花瓶溶液中的漆酶抑制剂足以维持非洲菊切花的水流过程和控制茎弯曲。