ABSTRACT

Drought is an environmental stress that limits agricultural and horticultural production worldwide. Paeonia lactiflora Pall. is a perennial flowering plant with strong environmental adaptability and a good candidate for production in arid areas. Using the Illumina Hiseq platform to study the transcriptome of P. lactiflora leaves under drought stress, 62.27 Gb of clean data was obtained and 87,099 unigenes (N50 = 1,352 bp; mean length = 790.66 bp) were obtained by transcriptome sequencing. After screening 4,198 differentially expressed genes, genes that may be related to the response of P. lactiflora to drought stress were identified: 28 signal transduction genes, 12 protein kinase genes, 14 transcription factor genes, 35 osmotic adjustment genes, 14 antioxidase enzyme genes, 22 photosynthesis genes, 9 AQP genes, 20 HSP genes, and 17 LEA genes. Transcriptomics analysis revealed that transcription factors, such as ERF, WRKY, and NAC transcription factor family members regulate the synthesis of hormones that play a role in signal transduction pathways of peony in response to drought stress. These results enrich currently available herbaceous peony gene sequence resources, fortify the identification of candidate genes and molecular regulatory mechanisms in response to drought stress, allowing genetic engineering to be used to create drought-tolerant plants.

摘要

干旱是一种环境压力，限制了全世界的农业和园艺生产。e药牡丹。是多年生开花植物，具有很强的环境适应能力，是干旱地区生产的良好候选植物。使用Illumina Hiseq平台研究干旱胁迫下的乳酸假单胞菌叶片转录组，获得了62.27 Gb的干净数据，并通过转录组测序获得了87,099个单基因（N50 = 1,352 bp；平均长度= 790.66 bp）。筛选了4,198个差异表达的基因后，这些基因可能与乳酸假单胞菌的反应有关确定了干旱胁迫：28个信号转导基因，12个蛋白激酶基因，14个转录因子基因，35个渗透调节基因，14个抗氧化酶基因，22个光合作用基因，9个AQP基因，20个HSP基因和17个LEA基因。转录组学分析表明，转录因子（例如ERF，WRKY和NAC转录因子家族成员）调节激素的合成，这些激素在响应干旱胁迫的牡丹信号传导途径中起作用。这些结果丰富了当前可用的草本牡丹基因序列资源，增强了候选基因的鉴定和响应干旱胁迫的分子调控机制，从而使基因工程可用于创建耐旱植物。