Abstract

A method for quantitative determination of the main active ingredient in traditional Chinese medicine was developed and validated, using liquid-liquid chromatography combined with high performance liquid chromatography, which was successfully applied to synephrine in Fructus Aurantii Immaturus. A biphasic solvent system composed of ethyl acetate - n-butanol - water - trifluoroacetic acid (1:2:3:0.003, v/v) was used to purify the crude extract of the samples by liquid-liquid chromatography. Then, quantitative analysis of synephrine in Fructus Aurantii Immaturus could be well determined by high performance liquid chromatography. Moreover, the collection time and retention time of synephrine could be well predicted using a continuous-stirred tank reactors model. Compared with previous studies, the present method showed advantages of good recovery, good repeatability, high selectivity to target components and simple operation.

摘要

开发并验证了一种定量测定中药主要有效成分的方法，该方法采用液-液色谱结合高效液相色谱法，已成功地应用于乌F中的辛弗林碱。由乙酸乙酯-正丁醇-水-三氟乙酸（1：2：3：0.003，v / v）组成的双相溶剂体系用于通过液-液色谱法纯化样品的粗提物。然后，定量分析未熟果中的辛弗林碱可以通过高效液相色谱法很好地确定。此外，使用连续搅拌釜式反应器模型可以很好地预测辛弗林的收集时间和保留时间。与以前的研究相比，本方法具有回收率好，重复性好，对目标组分的选择性高，操作简单等优点。